Protein Info for Atu0133 in Agrobacterium fabrum C58

Annotation: thymidine phosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 438 TIGR02643: thymidine phosphorylase" amino acids 3 to 437 (435 residues), 757.3 bits, see alignment E=5.1e-232 TIGR02644: pyrimidine-nucleoside phosphorylase" amino acids 6 to 420 (415 residues), 533.2 bits, see alignment E=4e-164 PF02885: Glycos_trans_3N" amino acids 6 to 68 (63 residues), 65.3 bits, see alignment E=5e-22 PF00591: Glycos_transf_3" amino acids 79 to 308 (230 residues), 163.3 bits, see alignment E=1.3e-51 PF07831: PYNP_C" amino acids 349 to 422 (74 residues), 75.4 bits, see alignment E=3.4e-25

Best Hits

Swiss-Prot: 100% identical to TYPH_AGRFC: Thymidine phosphorylase (deoA) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K00758, thymidine phosphorylase [EC: 2.4.2.4] (inferred from 100% identity to atu:Atu0133)

MetaCyc: 54% identical to thymidine phosphorylase (Escherichia coli K-12 substr. MG1655)
Pyrimidine-nucleoside phosphorylase. [EC: 2.4.2.2, 2.4.2.3]; Thymidine phosphorylase. [EC: 2.4.2.2, 2.4.2.3, 2.4.2.4]

Predicted SEED Role

"Thymidine phosphorylase (EC 2.4.2.4)" in subsystem Deoxyribose and Deoxynucleoside Catabolism (EC 2.4.2.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.2 or 2.4.2.3 or 2.4.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UJ08 at UniProt or InterPro

Protein Sequence (438 amino acids)

>Atu0133 thymidine phosphorylase (Agrobacterium fabrum C58)
MSLIPQEIIRRKRDGLSLAPQEIAAFIEALSKDGISEGQAAAFAMAVFFRGMNRDEMVAL
TLAMRDSGDVLSWRDIGRPVADKHSTGGVGDNVSLMLAPIVAACGLAVPMISGRGLGHTG
GTLDKLEAIPGYDVMPDEALFRRTVQSVGCAIIGQTGDLAPADKRLYAIRDVTATVDSIP
LITASILSKKLAAGLETLVLDVKVGNGAFMQSLEDARILARALVDVANGAGLPTTALITD
MNQPLCDAAGNAVEIVNCLEFLAGGKAGTRLEKVVLSFAAEMLVQARKAATLEEGEALAS
AALSSGRAMEIFARMVSVLGGPSDFIENPSRYLACAPIILPVPAARSGWLASCATRDLGM
VVVELGGGRTKPSDTINPAVGISDILPLGVRVEKGEPIAVVHAASSEDAERAVKRIEDCF
GIADNAPEIAASVLERIT