Protein Info for Atu0070 in Agrobacterium fabrum C58

Annotation: ribonucleoside-diphosphate reductase 2 alpha chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF08343: RNR_N
  • amino acids 1 to 76 (76 residues), 98.4 bits, E=2.9e-32
• TIGR04170: ribonucleoside-diphosphate reductase, class 1b, alpha subunit
  • amino acids 1 to 693 (693 residues), 1268.9 bits, E=0
• PF00317: Ribonuc_red_lgN
  • amino acids 78 to 150 (73 residues), 64.1 bits, E=1.5e-21
• TIGR02506: ribonucleoside-diphosphate reductase, alpha subunit
  • amino acids 82 to 675 (594 residues), 537.6 bits, E=4.1e-165
• PF02867: Ribonuc_red_lgC
  • amino acids 155 to 673 (519 residues), 551.6 bits, E=3e-169

Best Hits

Swiss-Prot: 83% identical to RIR1_MYCTO: Ribonucleoside-diphosphate reductase subunit alpha (nrdE) from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

KEGG orthology group: K00525, ribonucleoside-diphosphate reductase alpha chain [EC: 1.17.4.1] (inferred from 100% identity to atu:Atu0070)

MetaCyc: 72% identical to ribonucleoside-diphosphate reductase 2 subunit alpha (Escherichia coli K-12 substr. MG1655)
Ribonucleoside-diphosphate reductase. [EC: 1.17.4.1]; 1.17.4.1 [EC: 1.17.4.1]; 1.17.4.1 [EC: 1.17.4.1]; 1.17.4.1 [EC: 1.17.4.1]; 1.17.4.1 [EC: 1.17.4.1]

Predicted SEED Role

"Ribonucleotide reductase of class Ib (aerobic), alpha subunit (EC 1.17.4.1)" in subsystem Ribonucleotide reduction (EC 1.17.4.1)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (7/8 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (7/8 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (6/7 steps found)
• adenosine deoxyribonucleotides de novo biosynthesis I (2/2 steps found)
• guanosine deoxyribonucleotides de novo biosynthesis I (2/2 steps found)
• adenosine deoxyribonucleotides de novo biosynthesis II (3/4 steps found)
• guanosine deoxyribonucleotides de novo biosynthesis II (3/4 steps found)

KEGG Metabolic Maps

• Glutathione metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.17.4.1

Use Curated BLAST to search for 1.17.4.1

Sequence Analysis Tools

See Q7D293 at UniProt or InterPro

Protein Sequence (693 amino acids)

>Atu0070 ribonucleoside-diphosphate reductase 2 alpha chain (Agrobacterium fabrum C58)
MLNLYDENGKIQLDKDRLAARQYFLQHVNQNTVFFHNLREKLDYLVTEGYYEQEVLDQYS
FNFVRDLYDEAYSRKFRFPTFLGAFKYYTSYTLKTFDGKRYLERYEDRVCMVALALARGD
EQLARDLMDEIISGRFQPATPTFLNAGKKSRGELVSCFLLRVEDNMESIGRSINSALQLS
KRGGGVALSLTNIREFGAPIKQIENQSSGVIPVMKLLEDSFSYANQLGARQGAGAVYLHA
HHPDIMRFLDTKRENADEKIRIKTLSLGVVIPDITFELARNNEDMYLFSPYDVERVYGVP
LTEISVTEKYHEMVADSRIRKKKIKAREFFQVIAEIQFESGYPYIMFEDTVNRANPVAGR
ISMSNLCSEILQVSEASEFNADLSYSHMGKDISCNLGSLNIASAMDSADFGKTIETSIRA
LTAVSDMSHISSVPSVEKGNDASHAIGLGQMNLHGYLARERIFYGSEEGIDFTNIYFYTV
TYHAIRASNRLAVERGQSFKGFENSKYASGDYFDKYTDREWLPATEKVRDLFEKAGIAIP
TQEDWTALKQDVMASGLYNQNLQAVPPTGSISYINHSTSSIHPIVSKIEIRKEGKIGRVY
YPASYLSNDNLEYYQDAYEIGPEKIIDTYAAATQHVDQGLSLTLFFRDTATTRDINRAQI
YAWRRGIKTIYYIRLRQMALEGTQVQGCVSCTL