Protein Info for Atu0045 in Agrobacterium fabrum C58

Annotation: heat shock chaperone

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 435 TIGR00390: ATP-dependent protease HslVU, ATPase subunit" amino acids 5 to 435 (431 residues), 610.9 bits, see alignment E=7.2e-188 PF07728: AAA_5" amino acids 52 to 88 (37 residues), 22.2 bits, see alignment 2.4e-08 PF00004: AAA" amino acids 53 to 101 (49 residues), 26.8 bits, see alignment 1.3e-09 amino acids 211 to 324 (114 residues), 27.3 bits, see alignment E=9.2e-10 PF07724: AAA_2" amino acids 191 to 321 (131 residues), 89.4 bits, see alignment E=5.6e-29

Best Hits

Swiss-Prot: 100% identical to HSLU_AGRFC: ATP-dependent protease ATPase subunit HslU (hslU) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K03667, ATP-dependent HslUV protease ATP-binding subunit HslU (inferred from 100% identity to agr:AGROH133_02858)

Predicted SEED Role

"ATP-dependent hsl protease ATP-binding subunit HslU" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8UJ87 at UniProt or InterPro

Protein Sequence (435 amino acids)

>Atu0045 heat shock chaperone (Agrobacterium fabrum C58)
MTTFSPREIVSELDRHIIGQHDAKRAVAIALRNRWRRQQLDESLRDEVMPKNILMIGPTG
VGKTEISRRLAKLAGAPFIKVEATKFTEVGYVGRDVEQIIRDLVEIGIGLVREKKRAEVQ
AKAHQSAEERVLDALVGSTASPGTRESFRKKLRDGELDDKEIDIEVADSGSGMPGFEIPG
MPGANIGVLNLSEMFGKAMGGRTKKVRTTVSKSYTDLVRDESDKLLDNEMIQREAVKSVE
NDGIVFLDEIDKIAARDGGMGAGVSREGVQRDLLPLVEGTTVSTKYGPVKTDHVLFIASG
AFHVAKPSDLLPELQGRLPIRVELKPLTKEDFRRILTETEASLIRQYKALMATEELDLDF
TDDAIDALADVAVHLNSSVENIGARRLQTVMERVLDEISFNAPDRGGSAVKIDQEYVKKH
VGDLAADTDLSRYIL