Protein Info for Atu0033 in Agrobacterium fabrum C58

Annotation: two component sensor kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 596 transmembrane" amino acids 48 to 67 (20 residues), see Phobius details amino acids 280 to 302 (23 residues), see Phobius details PF13755: Sensor_TM1" amino acids 33 to 97 (65 residues), 98 bits, see alignment E=5.4e-32 PF13756: Stimulus_sens_1" amino acids 132 to 241 (110 residues), 126.9 bits, see alignment E=1.3e-40 PF00672: HAMP" amino acids 298 to 351 (54 residues), 30.1 bits, see alignment 1.3e-10 PF00512: HisKA" amino acids 359 to 422 (64 residues), 58.9 bits, see alignment E=1.1e-19 PF02518: HATPase_c" amino acids 476 to 591 (116 residues), 91.4 bits, see alignment E=1.3e-29

Best Hits

Swiss-Prot: 100% identical to CHVG_AGRFC: Sensor protein ChvG (chvG) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K14980, two-component system, OmpR family, sensor histidine kinase ChvG [EC: 2.7.13.3] (inferred from 100% identity to atu:Atu0033)

Predicted SEED Role

"Sensor histidine kinase ChvG (EC 2.7.3.-)" (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q07737 at UniProt or InterPro

Protein Sequence (596 amino acids)

>Atu0033 two component sensor kinase (Agrobacterium fabrum C58)
MLKKTPETVSDSDDAEERGSERRHRIHPLTIIRRIFGNAVFSSLTRRILFFNVAATVVLV
GGILYLNQFREGLIDARVESLLTQGEIIAGAVSASASVDTNSITINPEKLLELQAGQSIT
PAPNDEDLSFPINPERVAPVLRRLISPTRTRARLFDADANLLLDSRHLYSRGQVLRFDLP
PVTPETQTWGDWFTSMFNRMLQPSSLPQYKEAPGGDGSIYPEVMNALTGVRGAVVRVTEK
GELIVSVAVPVQRFRAVLGVLLLSTQAGDIDKIVHAERLAIMRVFGIATLVNIVLSLLLS
STIATPLRRLSAAAIRVRRGARTREEIPDFSARQDEIGNLSIALREMTTALYDRIDAIES
FAADVSHELKNPLTSLRSAVETLPRAKTEESKQRLTEIIFHDVRRLDRLISDISDASRLD
AELARADASPLDLDVLMKGLVDISRQISTKKKSVAIDYVADRKAGAKTSFVVNGHDLRIG
QIVTNLIENARSFVSEESGRITVRLSRHKDRCIVQVEDNGPGIQAEDIDRIFERFYTDRP
ASEGFGQNSGLGLSISRQIAEAHGGSLRAENVVDKYGVISGARFTLSLPAAETHER