Protein Info for AMB_RS23535 in Magnetospirillum magneticum AMB-1

Annotation: PAS domain S-box protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 1400 1510 PF01339: CheB_methylest" amino acids 12 to 187 (176 residues), 198.3 bits, see alignment E=4.4e-62 PF03705: CheR_N" amino acids 209 to 260 (52 residues), 50.9 bits, see alignment (E = 5.1e-17) PF01739: CheR" amino acids 274 to 462 (189 residues), 166.9 bits, see alignment E=1.9e-52 TIGR00229: PAS domain S-box protein" amino acids 826 to 950 (125 residues), 48.8 bits, see alignment E=7.7e-17 amino acids 956 to 1071 (116 residues), 73.8 bits, see alignment 1.3e-24 PF08448: PAS_4" amino acids 837 to 944 (108 residues), 50 bits, see alignment 1.7e-16 amino acids 957 to 1064 (108 residues), 33 bits, see alignment 3e-11 PF13426: PAS_9" amino acids 845 to 941 (97 residues), 27.5 bits, see alignment 1.5e-09 amino acids 962 to 1063 (102 residues), 74 bits, see alignment 5.3e-24 PF00989: PAS" amino acids 957 to 1062 (106 residues), 48.8 bits, see alignment 3.3e-16 PF08447: PAS_3" amino acids 973 to 1058 (86 residues), 41.4 bits, see alignment 7.2e-14 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 1072 to 1235 (164 residues), 165.1 bits, see alignment E=1.2e-52 PF00990: GGDEF" amino acids 1076 to 1232 (157 residues), 176.4 bits, see alignment E=1.9e-55 PF00563: EAL" amino acids 1254 to 1487 (234 residues), 246.6 bits, see alignment E=1.2e-76

Best Hits

KEGG orthology group: K13924, two-component system, chemotaxis family, CheB/CheR fusion protein [EC: 2.1.1.80 3.1.1.61] (inferred from 100% identity to mag:amb3329)

Predicted SEED Role

"Chemotaxis protein methyltransferase CheR (EC 2.1.1.80)" in subsystem Bacterial Chemotaxis (EC 2.1.1.80)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.80, 3.1.1.61

Use Curated BLAST to search for 2.1.1.80 or 3.1.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W1Z2 at UniProt or InterPro

Protein Sequence (1510 amino acids)

>AMB_RS23535 PAS domain S-box protein (Magnetospirillum magneticum AMB-1)
MDVKVDSKPHVIGIGASAGGLEALSQFVGGLPLDLGCIYVIAQHMSPTHRSMMADILSRE
TRLPVRELADGERPQPDVIYIIPPGTNLVFKDECFVLSQASPEISPKPSINILFQSMADE
FEERAIGIILSGTGSDGTRGLRAIKSAGGITFVQIPETAKYDGMPRSAIDACVADRIVSP
DQIGRELERLVRFPGMLPNLESGEQRPAELSDLFERVRLRTKIDFSSYKLSTVQRRLQRR
MAATNTGTLSEYLSKTDSDPDELDALAKETLISVTEFFRDKDAFRALERHAREIVSRKLP
GEEIRVWVVGCATGEEAYSLAIMFSELVSERGIGSRLQVFATDIDNNAMSVARRGIYNQT
AMAEMPQEYVSKYFIPCGNGFEPTKELRDCVTFARQDVNVDPPFLRLDLVTCRNVMIYFN
SDLQAKVLSILRYSLREDGLLFLGRSETVTQQEAMFASVDRRARIFRPRGQSRPITMGKL
VRGQLKVPRHEPRNPDRSNERLFLNALADHFGPAMLIDSGCRILHSHGPVSRFIHFPTGT
PEMNLAQLIVPEFSNEILTTMHRARRRQASSYSRKRRISSLDKQVWRLAVHPVGELAEAD
TFLVVFERSAQADAANAVKETEPQDGAEVFPDDELASTREHLQTLMEEMAASNEEMQALN
EEVQASNEELQASNEELEASNEELQATNEELVSVNEESLIKSAELAAINSDFEGVYNTID
FPIMVFDPELFLKRANGAAVRTYDLPLSASGMHIGRLKLPAFLDNIDKSLTAALTEQRKE
SFQITFGKRTFQVFVTPSMSMTGTPQSVVLVVVDHTDLVEAQEQIRESQERLLSIMNHST
SAVSLKDAAGRYEFINMRFEELFGLSAEEVIGKTDQQLFSRDIAQLLRSRDLEVMGQLSA
IQHLEEMVFPTSKVWLDSVRFPIFDSNGVVRAVCSQSTDVTMKHHAEEQLRLAAKVFDRA
GEAITITDSSGNIITVNDSFCRITGYSHQEVIGKNPRFLQSGKHGKDFYDAMWRSLIEQG
CWQGEIFNRRKNGEIYPEWLTVNSVRDENGSVVNYVAIFSDISAIKSSQRRIEFLATHDE
LTGIPNRSLLMDRLKHAVAQAKRQKTKLAVLFIDLDNFKVINDSLGHDVGDQLLKQATER
LKHCVRDSDTLARLGGDEFVAVLTDVSLEKVNSVAARIVDFLGASFSINDKSLFVSASIG
ISVFPDDGQDSLTLLKHADTAMYRAKERGRNQYQFFADEMKVIALQRLTLETGLRLALET
KSFRMCYQPQVDIHTGEVVGAEALLRWRDPTLGEVSPAHFIPIAESCGLIGAIGETVFTM
VLEQIAYWREKGLTVPRIAINVSAHQLRDAGFAAKVGSWLDSSGISSELIGIELTESALM
ERIDMVKEMLTSFDGMGMKVSIDDFGTGYSSLSYLKKLPIHELKIDRSFVDGIALERDDR
SIAKAIIDMSRALGMRVVAEGVESEEQLGVLKEEGCDMVQGYLFHRPLSPEAFAKVIETG
GKSSRKPLKR