Protein Info for AMB_RS22935 in Magnetospirillum magneticum AMB-1
Annotation: imidazole glycerol phosphate synthase subunit HisH
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 60% identical to HIS5_RHORT: Imidazole glycerol phosphate synthase subunit HisH (hisH) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)
KEGG orthology group: K02501, glutamine amidotransferase [EC: 2.4.2.-] (inferred from 100% identity to mag:amb4533)Predicted SEED Role
"Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-)" in subsystem Histidine Biosynthesis (EC 2.4.2.-)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
- L-histidine biosynthesis (10/10 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of plant hormones
- Purine metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.4.2.-
Use Curated BLAST to search for 2.4.2.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q2VYI8 at UniProt or InterPro
Protein Sequence (216 amino acids)
>AMB_RS22935 imidazole glycerol phosphate synthase subunit HisH (Magnetospirillum magneticum AMB-1) MTGIVAIIDYGSGNLRSAAKAFERVVAEASLNLSVAVTNDPKVVDRADRVVLPGVGAFAD CRAGLLALPGMAETLTERVLARGVPFFGICVGMQLMASIGREHGLHAGLGWVAGEVVPLG VEDQGLKVPHMGWNQLVPDGLSHPLLAGLDSEAHAYFVHSYKFEPANPAHRLAHVEYGGQ VTAVVGRDNMVGTQFHPEKSQITGLKVISNFLTWTP