Protein Info for AMB_RS22300 in Magnetospirillum magneticum AMB-1

Annotation: murein biosynthesis integral membrane protein MurJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 515 transmembrane" amino acids 26 to 45 (20 residues), see Phobius details amino acids 50 to 68 (19 residues), see Phobius details amino acids 88 to 110 (23 residues), see Phobius details amino acids 130 to 150 (21 residues), see Phobius details amino acids 157 to 179 (23 residues), see Phobius details amino acids 185 to 205 (21 residues), see Phobius details amino acids 229 to 254 (26 residues), see Phobius details amino acids 274 to 292 (19 residues), see Phobius details amino acids 313 to 336 (24 residues), see Phobius details amino acids 349 to 371 (23 residues), see Phobius details amino acids 383 to 402 (20 residues), see Phobius details amino acids 408 to 428 (21 residues), see Phobius details amino acids 448 to 469 (22 residues), see Phobius details amino acids 476 to 504 (29 residues), see Phobius details TIGR01695: murein biosynthesis integral membrane protein MurJ" amino acids 2 to 495 (494 residues), 430.7 bits, see alignment E=4.1e-133 PF03023: MurJ" amino acids 28 to 463 (436 residues), 398.3 bits, see alignment E=6e-123 PF13440: Polysacc_synt_3" amino acids 178 to 337 (160 residues), 23.1 bits, see alignment E=6.1e-09 PF01554: MatE" amino acids 246 to 398 (153 residues), 29.7 bits, see alignment E=7.3e-11

Best Hits

Swiss-Prot: 48% identical to MURJ_RHIME: Probable lipid II flippase MurJ (murJ) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K03980, virulence factor (inferred from 100% identity to mag:amb4409)

Predicted SEED Role

"Proposed peptidoglycan lipid II flippase MurJ" in subsystem ZZ gjo need homes

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2VYW2 at UniProt or InterPro

Protein Sequence (515 amino acids)

>AMB_RS22300 murein biosynthesis integral membrane protein MurJ (Magnetospirillum magneticum AMB-1)
MSLFRSIATIGGFTMLSRVTGLMREMMIAHFLGAGAVADAFFVAFRFPNLFRSLFAEGAF
NAAFVPLFTGKMTAEGTESARRFAEQSFAVLGLALALFVAVMELAMPWAIYGLAPGFDTV
PGKMALAVEFSRICFPYLLFISLVSLQAGVLNSMGRFAAAAATPVLLNLTSMAGLWFLVP
YSETAGHAMAWGTFAAGVVQFVWLSRAARRVGMGLGLVRPRLTPEVKLLFKRIVPGAVGA
GVYQVNLVINTMIASTVADGAVSYLNYADRVNQLPLGVVGIAIGTALLPTLSRQLKAGEA
EAARTSQNRAMELGLALTLPAAVALMVIAAPVIRVLFERGSFGPNETAATASALVAFAIG
LPAYVLVKVLVPGFFAREDTGTPVRVAGVAMVLNVVLNLSLAKPLGHVGMALATAIAAWA
NVLILAVLLARRGFFTVDERLKSKAPRILAACAVMGGVLWGGKLALWPLAQGQLMAVGVL
AGLVVLGAIAFLAAAQMLGALSLGEIKGMVRRKRG