Protein Info for AMB_RS22160 in Magnetospirillum magneticum AMB-1

Annotation: phenylalanine--tRNA ligase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 798 TIGR00472: phenylalanine--tRNA ligase, beta subunit" amino acids 1 to 797 (797 residues), 704 bits, see alignment E=1.3e-215 PF01588: tRNA_bind" amino acids 45 to 145 (101 residues), 74 bits, see alignment E=2.1e-24 PF03483: B3_4" amino acids 215 to 387 (173 residues), 217.2 bits, see alignment E=3.1e-68 PF03484: B5" amino acids 406 to 471 (66 residues), 67.6 bits, see alignment E=2.2e-22 PF17759: tRNA_synthFbeta" amino acids 477 to 683 (207 residues), 152.5 bits, see alignment E=2.9e-48 PF03147: FDX-ACB" amino acids 704 to 797 (94 residues), 84 bits, see alignment E=2e-27

Best Hits

Swiss-Prot: 100% identical to SYFB_MAGSA: Phenylalanine--tRNA ligase beta subunit (pheT) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K01890, phenylalanyl-tRNA synthetase beta chain [EC: 6.1.1.20] (inferred from 100% identity to mag:amb4380)

Predicted SEED Role

"Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2VYZ1 at UniProt or InterPro

Protein Sequence (798 amino acids)

>AMB_RS22160 phenylalanine--tRNA ligase subunit beta (Magnetospirillum magneticum AMB-1)
MKFTLDWLKSHLDTDATLEQIEVGLTAIGLEVEGIDDPSKHLGGFVVGHILEAEKHPDAD
KLKLCKVDSGAGILQVVCGAPNARAGLKVILAQPGTYIPITGDVLKKGKVRGVESQGMMC
SWRELKLGEDHDGIAELDVTLAVGASLLDIMSFDPMIEISVTPNRVDCLGVRGIARDLAA
FGLGNLKPLKVEPVPGAFKSSIGVRLEFAPEDSNACPLFAGRLIRGVKNGDSPQWLKDRL
TAIGLRPISALVDITNFFAYDLCRPLHVFDAAKVKGDIRARLARDGETLAGLNGKTYTLE
SGMTVIADENGPEALAGILGGEHSGCTEATTEVFLEAAYFDPIRTAATGRKLDILSDARF
RFERGVDPAFVVPSMELATRMILDLCGGEASEAVIAGTEPDWQKSIVLRPNRVAELGGVE
VSTQRMETILNDLGCAVAEHADGLLVNPPSWRGDITAEHDLVEEVIRINGYDNIPATPMP
RPAMPKPVLTPGQRRSGWVRRQLATRGLVETVTWSFLPEAQAVMFGGGAPEMHLANPISS
DLDVMRPSVLPNLVTAAGRNADRGMKDLGLFELGPQFDGPEPGQQRLVAAGIRAGRARGR
HWADPARAVDAFDAKADILSAVAAAGANPDSLQVVAEAPVWYHPGRSGTLKLGNKPVGFF
GEIHPGLLAKLDVKGPVAGFELFLEALPAQKAKATKAKPLLKASALQPLDRDFAFTLDTG
VAADAVVRAARNADKVLISDVAVFDLYEGDKMAAGKKSLAITVTLQPTDKTLTDEDIEAV
GAKIVAAVAKATGGELRG