Protein Info for AMB_RS20340 in Magnetospirillum magneticum AMB-1
Annotation: asparagine synthase (glutamine-hydrolyzing)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01953, asparagine synthase (glutamine-hydrolysing) [EC: 6.3.5.4] (inferred from 100% identity to mag:amb4020)Predicted SEED Role
"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)" in subsystem Cyanophycin Metabolism or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)
MetaCyc Pathways
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- L-asparagine biosynthesis I (1/1 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-citrulline biosynthesis (6/8 steps found)
- superpathway of L-asparagine biosynthesis (1/2 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
- L-glutamate and L-glutamine biosynthesis (4/7 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.5.4
Use Curated BLAST to search for 6.3.5.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q2W001 at UniProt or InterPro
Protein Sequence (635 amino acids)
>AMB_RS20340 asparagine synthase (glutamine-hydrolyzing) (Magnetospirillum magneticum AMB-1) MCGFAGFLDLTRATPDRETVVRAMAATLVHRGPDDDGAWVDDDAGVALGFRRLAIIDLTP QGHQPMTSHDGRWVISFNGEIYNHADLRARLGESMAWRGHSDTEVLLEAVSAWGIEAALA AFDGMFAFALWDRRERVLTLARDRFGEKPLYWAKAGGSLLFGSELKALRAHPAWTGGIDR DALALYMRLGWIPAPHTIHPGVFKLEPGHLMRVNGGDISAKAYWSAADCAAGLEDSFAGS AEDAADRLEELLRESVRLRMQADVPVGVFLSGGIDSSATAALMRQLSAEPVHSFTIGFDQ AGLDESAHARAVADHLGTLHTEVRISDAEALAQVARLPVLYDEPFADAAALPTALLAQVT RKSVTVALSGDGADELFGGYGIYRSIPRDWRRLQGLPDGLRALGAMGARGLAGPAEGLAA LAGTVRKRRSHPGHRLGREAERLEAGSLAELLGLHYSRWRGMSDLVPGAGRAESLFSAPT PRLHDDALATMVLDAMGYLPDDLCVKTDRATMGASLEARLPFLALDIARFAWSLPTSLKI EGETGKAVLRRVLYRLVPRALVDRPKMGFEVPLRRWLNGPLRDWADDLLSEERLRRQGHL NAALVAQCWREHRSGAKNWQNEMWHALMFQAWLEH