Protein Info for AMB_RS20235 in Magnetospirillum magneticum AMB-1

Annotation: HlyD family secretion protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 343 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF00529: CusB_dom_1" amino acids 7 to 338 (332 residues), 38.6 bits, see alignment E=1.7e-13 PF16576: HlyD_D23" amino acids 43 to 286 (244 residues), 74.9 bits, see alignment E=1.1e-24 PF13533: Biotin_lipoyl_2" amino acids 45 to 90 (46 residues), 39.5 bits, see alignment 7.6e-14 PF13437: HlyD_3" amino acids 209 to 328 (120 residues), 61.5 bits, see alignment E=2.4e-20

Best Hits

KEGG orthology group: K03543, multidrug resistance protein A (inferred from 100% identity to mag:amb3998)

Predicted SEED Role

"Membrane fusion component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W023 at UniProt or InterPro

Protein Sequence (343 amino acids)

>AMB_RS20235 HlyD family secretion protein (Magnetospirillum magneticum AMB-1)
MKKPIAIAAALVLVSFGSWSAYRWSQEWRWMESTDDAYVDGDITTISPKVAGHVVELLAR
DNRAVAKGDVLVRIDERDYRARLDEAAGQVKARLAQLIQIDDRVAVQEAVITQSGASISA
ARAEMVRAKADYERSRALVREDYVSRQRFDITQADAARAEAGLTGSGAGLQAARRQLSVL
ASERNVALAQLEQAKATLVVAETELEATTIRSPVDGVIGNRAVRDGQYVRPGQTLLAVVP
LGNVWIDANFKETQIGRMTPGNRVEIKVDAFPGAVIGGSVDSFAPASGAKFSLLPPENAT
GNFTKVVQRIPVRIRVDQDSPLAGQLRPGLSVVVKVDTRDQGK