Protein Info for AMB_RS20125 in Magnetospirillum magneticum AMB-1

Annotation: CoA ester lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03328: HpcH_HpaI
  • amino acids 30 to 273 (244 residues), 89 bits, E=1.4e-29

Best Hits

Swiss-Prot: 71% identical to MCLA_CHLAU: Malyl-CoA/beta-methylmalyl-CoA/citramalyl-CoA lyase (mcl) from Chloroflexus aurantiacus

KEGG orthology group: K08691, malyl-CoA lyase [EC: 4.1.3.24] (inferred from 100% identity to mag:amb3977)

MetaCyc: 71% identical to L-malyl CoA lyase subunit (Chloroflexus aurantiacus)
Citramalyl-CoA lyase. [EC: 4.1.3.25]; Malyl-CoA lyase. [EC: 4.1.3.25, 4.1.3.24]; 4.1.3.24 [EC: 4.1.3.25, 4.1.3.24]

Predicted SEED Role

"L-malyl-CoA/beta-methylmalyl-CoA lyase (EC 4.1.3.-)" (EC 4.1.3.-)

MetaCyc Pathways

• methylaspartate cycle (14/19 steps found)
• superpathway of the 3-hydroxypropanoate cycle (13/18 steps found)
• 3-hydroxypropanoate cycle (9/13 steps found)
• glyoxylate assimilation (9/13 steps found)
• ethylmalonyl-CoA pathway (7/11 steps found)
• formaldehyde assimilation I (serine pathway) (8/13 steps found)
• itaconate degradation (1/4 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (5/14 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• C5-Branched dibasic acid metabolism
• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.-

Use Curated BLAST to search for 4.1.3.- or 4.1.3.24 or 4.1.3.25

Sequence Analysis Tools

See Q2W044 at UniProt or InterPro

Protein Sequence (349 amino acids)

>AMB_RS20125 CoA ester lyase (Magnetospirillum magneticum AMB-1)
MSVKPPRKFFEPLAIGAPAPYRELPVRLERMIHFFPPHNEKMRAKAAEMGKNVDVLLGNL
EDAIPADAKDAARAGFVEVAKAWDNPNTGLWTRVNCLNSPWFLDDMNAIVGEAGNKVDVV
MLPKVEGPWDIHYLDQLLAQLEAKHGVKRPIMIHAILETALGVENVAAICQASPRMHGIS
LGPADLAASRGMKTTRVGGGHPFYGVLEDAAEGKAGRTLFQQDLWHYTVAKMVDACQSAG
IKAFYGPFGDFSDDAACEAQFRNAFLLGCAGGWSLHPKQIDIAKKVFSPDVAEVLFAKKI
LEAMPDGTGAVMIDGKMQDDATWKQAKVMVDLAKAVAAKDPAMAQAYGL