Protein Info for AMB_RS20100 in Magnetospirillum magneticum AMB-1
Annotation: homoserine O-acetyltransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 74% identical to METXA_RHORT: Homoserine O-acetyltransferase (metXA) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)
KEGG orthology group: K00641, homoserine O-acetyltransferase [EC: 2.3.1.31] (inferred from 100% identity to mag:amb3972)MetaCyc: 40% identical to serine O-acetyltransferase (Streptomyces lavendulae lavendulae)
Serine O-acetyltransferase. [EC: 2.3.1.30]
Predicted SEED Role
"Homoserine O-acetyltransferase (EC 2.3.1.31)" in subsystem Methionine Biosynthesis (EC 2.3.1.31)
MetaCyc Pathways
- superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
- superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
- superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
- L-cysteine biosynthesis I (2/2 steps found)
- L-homocysteine biosynthesis (2/2 steps found)
- L-methionine biosynthesis III (3/4 steps found)
- superpathway of L-cysteine biosynthesis (fungi) (4/6 steps found)
- L-cysteine biosynthesis VII (from S-sulfo-L-cysteine) (2/4 steps found)
- seleno-amino acid biosynthesis (plants) (2/5 steps found)
- N-3-oxalyl-L-2,3-diaminopropanoate biosynthesis (1/4 steps found)
- L-cysteine biosynthesis VI (reverse transsulfuration) (2/7 steps found)
- D-cycloserine biosynthesis (1/6 steps found)
- superpathway of seleno-compound metabolism (5/19 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.3.1.30, 2.3.1.31
Use Curated BLAST to search for 2.3.1.30 or 2.3.1.31
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q2W049 at UniProt or InterPro
Protein Sequence (393 amino acids)
>AMB_RS20100 homoserine O-acetyltransferase (Magnetospirillum magneticum AMB-1) MTQSEPVPTASANPAGLKVELGRDRPIRLDCGVELGPIQVAYQTYGRLNADKSNAILICH ALTGDHYVADPHPITAKPGWWHELVGPGRVFDTDRYFLICSNVLGGCMGTTGPVDENPAT GQAWGLDFPVITIGDMVKVQARLVDHLGIDQLFCVVGGSMGGMQVLKWAQVYPERVFSAI PIASAARHSAQNIAFHEVGRQAIMADPDWCEGNYLQEGKRPHRGLAVARMAAHITYLSEP ALHQKFGRNLQNRDTVTYGFDADFQVESYLRHQGNTFVDRFDANSYLYITRAMDYFDLAA ESGGVLANAFRGTRTRFCVVSFTSDWLFPTPESRAVVHALNAVAANVSFVEIKSDKGHDA FLLDEPEFHATLTGFLEGAATHRGLPRTEGAGR