Protein Info for AMB_RS20040 in Magnetospirillum magneticum AMB-1

Annotation: 2-oxoglutarate dehydrogenase E1 component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 988 PF16078: 2-oxogl_dehyd_N" amino acids 8 to 47 (40 residues), 59.4 bits, see alignment (E = 4e-20) TIGR00239: oxoglutarate dehydrogenase (succinyl-transferring), E1 component" amino acids 10 to 959 (950 residues), 1131.7 bits, see alignment E=0 PF00676: E1_dh" amino acids 235 to 551 (317 residues), 184.5 bits, see alignment E=4.8e-58 PF02779: Transket_pyr" amino acids 620 to 814 (195 residues), 163.3 bits, see alignment E=9.3e-52 PF16870: OxoGdeHyase_C" amino acids 819 to 964 (146 residues), 174.2 bits, see alignment E=3.1e-55

Best Hits

Swiss-Prot: 60% identical to ODO1_BRUC2: 2-oxoglutarate dehydrogenase E1 component (sucA) from Brucella canis (strain ATCC 23365 / NCTC 10854)

KEGG orthology group: K00164, 2-oxoglutarate dehydrogenase E1 component [EC: 1.2.4.2] (inferred from 100% identity to mag:amb3961)

Predicted SEED Role

"2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2)" in subsystem TCA Cycle (EC 1.2.4.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W060 at UniProt or InterPro

Protein Sequence (988 amino acids)

>AMB_RS20040 2-oxoglutarate dehydrogenase E1 component (Magnetospirillum magneticum AMB-1)
MTKQFDESSFLSGGNAVFIAELYTRYLEDPASVDSSWVSFFQDLKDDGSQLVQDFKGTAS
ARRDLKIIGAVDPEAAAAAAAAAKKGGKDAGKGAAAPAADPAAIRQGQIDSIRALMLIRS
YRVRGHLMAQLDPLGLSKPEQHPELDYRTYGFTDADLDREIFIDHVLGLESASLRTIVRI
VQETYCARIGVEFMHIQDPDQKAWIQKRIESIHNRTDFTARGKTAILERLTEAEGFERFL
QMKYTGTKRFGLEGGESVIPALEQILKRGSQLGVDEVVMGMAHRGRLNVLANFMKKPYQA
IFSEFQGNAANPEDVQGSGDVKYHLGTSADRDFDGKTVHLSLMPNPSHLEVVGPLVVGKV
RAKQTQFGDTERKRVMGIILHGDAAFAGQGVVPETMLLSQLKGYATGGTMHIIINNQIGF
TTAPQYSRSGPHSSDVAKGFQAPVFHVNGDDPEAVVHVARIATEYRQEFGADVVIDMVCY
RRHGHNESDEPAFTQPQMYRKIASHPTTRAIYMEKLVAEGTLSRYDADAIFANFQARLEQ
DYEAAKSFKVNKADWLEGKWQGLAQLAEEEEFREEKTGVAADILKEVGHALARTPEGFNV
NKKIVRQLQAKKEMMDKGEGIDWATAEALAFGTLLIEGNGVRLSGQDCGRGTFSQRHCRL
TDQETEDRVEPLNHIRPGNQAYFEVMDSPLSEEAVLGFEYGYSQAEPNTLTLWEGQFGDF
ANGAQVIIDQFINSGESKWLRMSGLVMLLPHGYEGQGPEHSSARWERYLQLSGEDNWQVC
NITTPANYFHALRRQLRRNFRKPLIIMTPKSLLRHKLCVSKLDDLVTGSRFRRVLPETET
LVADAKIRRVLLCSGKVYYDLLEERTKRGLKDVAIIRVEQLYPWPKDTIKAQLARYPNAE
LLWVQEEPANMGPWTFVDRRIEFICEELDIKAKKALYCGRRAAASPATGLYKTHVAEQEW
ITGMALTGELVTLPQPFRRASKLSRLTA