Protein Info for AMB_RS19870 in Magnetospirillum magneticum AMB-1

Annotation: shikimate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 191 PF13671: AAA_33" amino acids 20 to 132 (113 residues), 30.2 bits, see alignment E=7.9e-11 PF01202: SKI" amino acids 26 to 177 (152 residues), 147.8 bits, see alignment E=4.6e-47

Best Hits

Swiss-Prot: 100% identical to AROK_MAGSA: Shikimate kinase (aroK) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K00891, shikimate kinase [EC: 2.7.1.71] (inferred from 100% identity to mag:amb3926)

MetaCyc: 40% identical to shikimate kinase 1 (Escherichia coli K-12 substr. MG1655)
Shikimate kinase. [EC: 2.7.1.71]

Predicted SEED Role

"Shikimate kinase I (EC 2.7.1.71)" in subsystem Benzoate transport and degradation cluster or Chorismate Synthesis or Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) (EC 2.7.1.71)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.71

Use Curated BLAST to search for 2.7.1.71

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W095 at UniProt or InterPro

Protein Sequence (191 amino acids)

>AMB_RS19870 shikimate kinase (Magnetospirillum magneticum AMB-1)
MADELARLAPLLSGRIGRTIVLVGLMGAGKSCVGRRLAARLGLDFVDSDAEFEAASGSSI
SDYFARFGEAAFREGERKVIARLLDGPPVVLATGGGAFVDPTTRERIKAAGTSVWIRADL
ELLLKRTVGRDHRPLLKQGDPREILGRLMEARYPIYAEADIIVESTDEVPEATVIRVMEG
LITYLDLEKTV