Protein Info for AMB_RS19670 in Magnetospirillum magneticum AMB-1

Annotation: metal-transporting ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 869 transmembrane" amino acids 62 to 80 (19 residues), see Phobius details amino acids 86 to 102 (17 residues), see Phobius details amino acids 251 to 270 (20 residues), see Phobius details amino acids 277 to 305 (29 residues), see Phobius details amino acids 678 to 696 (19 residues), see Phobius details amino acids 702 to 722 (21 residues), see Phobius details amino acids 744 to 764 (21 residues), see Phobius details amino acids 776 to 795 (20 residues), see Phobius details amino acids 809 to 830 (22 residues), see Phobius details amino acids 843 to 861 (19 residues), see Phobius details PF00690: Cation_ATPase_N" amino acids 10 to 76 (67 residues), 63.3 bits, see alignment 3.9e-21 PF00122: E1-E2_ATPase" amino acids 117 to 310 (194 residues), 171.8 bits, see alignment E=3.2e-54 TIGR01494: HAD ATPase, P-type, family IC" amino acids 222 to 351 (130 residues), 81.3 bits, see alignment E=2.6e-27 amino acids 581 to 693 (113 residues), 100.5 bits, see alignment E=4e-33 PF00702: Hydrolase" amino acids 328 to 623 (296 residues), 64.5 bits, see alignment E=5.5e-21 PF13246: Cation_ATPase" amino acids 374 to 455 (82 residues), 53.1 bits, see alignment E=7.9e-18 PF00689: Cation_ATPase_C" amino acids 694 to 865 (172 residues), 174.1 bits, see alignment E=7.3e-55

Best Hits

KEGG orthology group: K01537, Ca2+-transporting ATPase [EC: 3.6.3.8] (inferred from 100% identity to mag:amb3888)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5 or 3.6.3.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W0D3 at UniProt or InterPro

Protein Sequence (869 amino acids)

>AMB_RS19670 metal-transporting ATPase (Magnetospirillum magneticum AMB-1)
MPHSDGEPVWHRLGADAVRARLGCGTEGLAEAEILARRGRCGLNRLPEKPPRPAWAVFLD
QFRNLLTLMLLAAGVLAGLVGDTSDMVVVLAVTLFNAFLGYAQERRAGRMLGALKAMLAQ
KARVRRAAGEAVIAAEGLVPGDLVLLKAGDRVPADGRLVDGQDVEADEAALTGESEPVAK
ITGALDRDDTPLAERVNMLFMNSVITRGRAEMAVTGTGRLSEMGKVAALLDAAEDGPTPL
QQRLDQLGRRLALIAAIVVGFVLVQGLLAGEPLGRMVLTAVALAVAAIPEGLPAVVTVTL
ALGMVRMAAKGAVVRRLAAVETLGSTSVICSDKTGTLTLGRMTALEGWALGRSHGFAGDD
RPVGLEWGLAPAGLCTEARFNAATGEPVGDPTEVALLRLAERAGALPPPGRWRRLAEVPF
SSASKLMATLDGDGETCRLSVKGAPDRVLELCDTVLTEDGARPLDAEQRRVLAAEMERMG
GRALRVIALASRPAQPDEDLGAGLGGLVLHALVGLADPPRPGAADAIATCRAAGIMVKMI
TGDHRVTAAAVARQLGLDGEAVDGGELDGLSDVELARRVEAIAVFARVTPEHKVRIVRAL
KACGHVTAMTGDGVNDAAALRTAHIGVAMGRTGSDVTREAAAMVLTGDDFSTVVGAVREG
RIITDNIVKFVRFQLSTNMGALLAVLLAPLCGLPVPFSPIQILWVNIIMDGPPAMALAFD
PARRGLMDQPPRPQNASILPLSRLLRLFSFGVAMALGTLGVLWWEGGGGDMVRARTMAFT
TFVLFQVFNVFNARVGAESAFGRAALANAKLWGALAGIVALQVVAVHWAPAQALFHTTDL
DPAEWGIAAALGASVLVLEELRKLVRRLS