Protein Info for AMB_RS19545 in Magnetospirillum magneticum AMB-1

Annotation: elongation factor 4

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 TIGR01393: elongation factor 4" amino acids 6 to 597 (592 residues), 979.6 bits, see alignment E=5.1e-299 PF00009: GTP_EFTU" amino acids 6 to 184 (179 residues), 185.8 bits, see alignment E=1.6e-58 TIGR00231: small GTP-binding protein domain" amino acids 9 to 176 (168 residues), 71.7 bits, see alignment E=6.2e-24 PF03144: GTP_EFTU_D2" amino acids 208 to 278 (71 residues), 36 bits, see alignment E=2e-12 PF00679: EFG_C" amino acids 402 to 486 (85 residues), 76.2 bits, see alignment E=4.3e-25 PF06421: LepA_C" amino acids 490 to 596 (107 residues), 169 bits, see alignment E=7.2e-54

Best Hits

Swiss-Prot: 100% identical to LEPA_MAGSA: Elongation factor 4 (lepA) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K03596, GTP-binding protein LepA (inferred from 100% identity to mag:amb3862)

MetaCyc: 61% identical to 30S ribosomal subunit biogenesis factor LepA (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Translation elongation factor LepA" in subsystem Heat shock dnaK gene cluster extended or Universal GTPases

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W0F9 at UniProt or InterPro

Protein Sequence (600 amino acids)

>AMB_RS19545 elongation factor 4 (Magnetospirillum magneticum AMB-1)
MTKLSHIRNFSIVAHIDHGKSTLADRLIQACGAIEAREMKEQMLDSMDIERERGITIKAQ
TVRLDYKAKDGKTYQLNLMDTPGHVDFAYEVSRSLAACEGSLLVVDASQGVEAQTLANVY
HALDAGHEVVPVLNKIDLPAAEPERVKQQIEDVIGLDTSDAVMISAKTGIGIGDVLEALV
NRLPCPEGDADAPLQALLVDSWYDPYLGVIILVRIKNGVLKKGQKIRMMATQAAYQVDSC
GYFTPKLVQTTELRPGEMGFFTAAIKAVADTKVGDTVTDDKKPADTPLPGFKPSVPVVWC
GLFPIDAADYEDLRDSLAKLRLNDASFQYEPETSAALGFGFRCGFLGLLHLEIIQERLER
EFNLDLITTAPSVVYRVHKTNGEMEELHNPADMPDPARIDHMEEPWIKATIMVPDEYLGP
VLTLCTERRGQQIDLTYAGSRAMAVYKLPLNEVVFDFYDRLKSISRGYASFDYEVDSYAQ
SDLVKVSILVNAEPVDALSFVVHRANAESRGRGICTRLKELIPRQMFQIAIQAAIGGRIV
ARESISALRKDVTAKCYGGDATRKRKLLEKQKEGKKRMRQFGKVEIPQSAFLAALKMGDS