Protein Info for AMB_RS18485 in Magnetospirillum magneticum AMB-1

Annotation: aconitate hydratase AcnA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01341: aconitate hydratase 1
  • amino acids 21 to 900 (880 residues), 1260.7 bits, E=0
• PF00330: Aconitase
  • amino acids 76 to 565 (490 residues), 522.3 bits, E=1.3e-160
• PF00694: Aconitase_C
  • amino acids 694 to 827 (134 residues), 128.7 bits, E=1.8e-41

Best Hits

KEGG orthology group: K01681, aconitate hydratase 1 [EC: 4.2.1.3] (inferred from 67% identity to eba:ebA5264)

Predicted SEED Role

"Aconitate hydratase (EC 4.2.1.3)" in subsystem Propionate-CoA to Succinate Module or Serine-glyoxylate cycle or TCA Cycle (EC 4.2.1.3)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (20/22 steps found)
• TCA cycle V (2-oxoglutarate synthase) (9/9 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• TCA cycle III (animals) (9/10 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• TCA cycle VII (acetate-producers) (8/9 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (20/26 steps found)
• reductive TCA cycle I (9/11 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (7/9 steps found)
• superpathway of glyoxylate bypass and TCA (9/12 steps found)
• methylaspartate cycle (14/19 steps found)
• partial TCA cycle (obligate autotrophs) (6/8 steps found)
• ethene biosynthesis V (engineered) (18/25 steps found)
• glyoxylate cycle (4/6 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• reductive TCA cycle II (8/12 steps found)
• nitrogen remobilization from senescing leaves (5/8 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (9/14 steps found)
• mixed acid fermentation (9/16 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.3

Use Curated BLAST to search for 4.2.1.3

Sequence Analysis Tools

See Q2W120 at UniProt or InterPro

Protein Sequence (903 amino acids)

>AMB_RS18485 aconitate hydratase AcnA (Magnetospirillum magneticum AMB-1)
MLPDPFGALRDLSLGHERLSRYYALAALDAVTDGPVSRLPVSIRIVLESVLRNCDGKRIT
EEHVRQLANWRPDAPRTQEIPFVVARIVLQDFTGVPLLCDLAAMRGVAQAFGKNPKIIEP
LVPVDLVVDHSVQVDHYGEADSLDLNMRREFQRNAERYRFIKWGMQAFDTFRVVPPGIGI
VHQVNLEFLARGVLEKDGITYPDTLVGTDSHTTMINALGVAGWGVGGIEAEAGMLGQPLV
FLTPDVVGVHLHGRLPEGATATDLVLFLTERLRRAKVVGKFVEFFGEGTRSLAVPDRATI
ANMAPEYGATMGFFPVDKETVRYLEATGRTDSEIEVFRAYYSAQGLFGMPMPGDIDYSEV
IEFDLGSVQPSIAGPKRPQDRLNLSDMRRAFTSLFSAPAKDDGYGRPAEALGRRHRVETT
AAADIGHGDVLIAAITSCTNTSNPGVMLAAGLLARKAVALGLKVGPRVKTSLAPGSRVVT
EYLAKAGLLGDLESLGFGVVAYGCTTCIGNSGPLMPDLEQAIAADDLVCAAVLSGNRNFE
ARIHPAIKANFLMSPPLVVAFAIAGRIAIDMTQEPLGTGKDGKPVMLKDIWPSGREVADA
LLVATDPELYRRLYSDFVHGNPLWNDIPTQTGPAYAWETSTYIAEPPFFERFSPQPAGVG
DIIGARALAIFGDSVTTDHISPAGSIAVSSPAGQYLLAHGVAAGDFNSYGARRGNHEVMM
RGTFANVRIRNLMLPAKVDGSRVEGGLTLHQPEGSEMPIFDAASRYQEAGIPSIVFAGTE
YGTGSSRDWAAKGPKLLGVRAVVAQSFERIHRSNLVGMGVLPLQFRDGESAASLGIAGDE
EFHVRGLSGVLRPRQEVVLEIVNRQGRSRAISLQLRVDTAIELDYLSHGGILPYVLRDLL
AAG