Protein Info for AMB_RS18050 in Magnetospirillum magneticum AMB-1

Annotation: sulfate ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 338 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details TIGR00971: sulfate ABC transporter, sulfate-binding protein" amino acids 30 to 334 (305 residues), 415.4 bits, see alignment E=7.5e-129 PF13531: SBP_bac_11" amino acids 40 to 289 (250 residues), 138.4 bits, see alignment E=6.6e-44 PF01547: SBP_bac_1" amino acids 60 to 281 (222 residues), 45.5 bits, see alignment E=2.1e-15 PF13416: SBP_bac_8" amino acids 82 to 286 (205 residues), 27.3 bits, see alignment E=6e-10 PF13343: SBP_bac_6" amino acids 138 to 321 (184 residues), 33.6 bits, see alignment E=5.8e-12

Best Hits

Swiss-Prot: 55% identical to CYSP_SALTY: Thiosulfate-binding protein (cysP) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02048, sulfate transport system substrate-binding protein (inferred from 100% identity to mag:amb3567)

MetaCyc: 55% identical to thiosulfate/sulfate ABC transporter periplasmic binding protein CysP (Escherichia coli K-12 substr. MG1655)
ABC-19-RXN [EC: 7.3.2.5]; ABC-7-RXN [EC: 7.3.2.5, 7.3.2.3]; 7.3.2.3 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-478 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-479 [EC: 7.3.2.5, 7.3.2.3]

Predicted SEED Role

"Sulfate and thiosulfate binding protein CysP" in subsystem Cysteine Biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.3 or 7.3.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W1A4 at UniProt or InterPro

Protein Sequence (338 amino acids)

>AMB_RS18050 sulfate ABC transporter substrate-binding protein (Magnetospirillum magneticum AMB-1)
MALRFPTLAVLAALALVPAGASAEDKVPGELLNVSYDIARELFQQINPAFQATPAGKLAD
GKAIEIKQSHAGSSKQARAILEGLPADVVTFNQVTDVQVLADKGFVRKDWQTAFPSGASP
YYSLPAFVVRKGNPKNIRNWDDLVRPDVKVVFPNPKTSGNGRYTYLAAYAFALEAAKGDQ
VQAKEFIRKLVANVPVFDTGGRGATTTFVERATGDVLVTFEAEVGGVIKEFGADKVEGVI
PPVSILAEFPVAVVDKVAAKKGSAKTAKAYLDFLYSDDGQEILAKNFYRVRSEKVAARHA
AAFPKVRLYTTELFGGWDNIQKIHFAEGGIFDQVFGKK