Protein Info for AMB_RS17735 in Magnetospirillum magneticum AMB-1

Annotation: tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00174: tRNA dimethylallyltransferase
  • amino acids 8 to 291 (284 residues), 208.1 bits, E=8.5e-66
• PF01715: IPPT
  • amino acids 43 to 284 (242 residues), 221.9 bits, E=1.1e-69

Best Hits

Swiss-Prot: 100% identical to MIAA_MAGSA: tRNA dimethylallyltransferase (miaA) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K00791, tRNA dimethylallyltransferase [EC: 2.5.1.75] (inferred from 100% identity to mag:amb3506)

Predicted SEED Role

"tRNA dimethylallyltransferase (EC 2.5.1.75)" (EC 2.5.1.75)

MetaCyc Pathways

• cis-zeatin biosynthesis (1/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Zeatin biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.75

Sequence Analysis Tools

See Q2W1G5 at UniProt or InterPro

Protein Sequence (315 amino acids)

>AMB_RS17735 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA (Magnetospirillum magneticum AMB-1)
MTSTSTPAVVIAGPTASGKSGLAVRIAREFGGVVINADSMQVTDALPLLTARPAPGDMAL
APHRLYAVLPPTELCSAARWRDMAAMEMAAAWQAGMLPILTGGTGLYLKAVMEGLSPIPE
IPESIRAEARTLLAEMGNAAFHNRLAKHDPLMAERLDSGNSQRLARAWEVVTATGRSLAE
WQDEPREGAVEARWFSLVLDPERPRLYAQCESRFRAMVTAGALDEVRDFEALRLPPDLPI
QKALGRRELAAHLAGETDLDTAIAAACQATRNYAKRQGTWFRHQMMASLTASEQLSESLI
ATIFLKIRQHLLTAP