Protein Info for AMB_RS17475 in Magnetospirillum magneticum AMB-1

Annotation: NADPH-dependent 2,4-dienoyl-CoA reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 666 PF00724: Oxidored_FMN" amino acids 6 to 330 (325 residues), 261.3 bits, see alignment E=7e-81 PF00070: Pyr_redox" amino acids 376 to 410 (35 residues), 22.1 bits, see alignment (E = 8e-08) PF03486: HI0933_like" amino acids 376 to 414 (39 residues), 31.4 bits, see alignment 3.9e-11 PF01266: DAO" amino acids 376 to 411 (36 residues), 33.5 bits, see alignment 1.6e-11 PF07992: Pyr_redox_2" amino acids 376 to 632 (257 residues), 95.3 bits, see alignment E=2.1e-30 PF12831: FAD_oxidored" amino acids 377 to 413 (37 residues), 33.5 bits, see alignment 1.4e-11 PF00890: FAD_binding_2" amino acids 377 to 414 (38 residues), 29.3 bits, see alignment 2.4e-10 PF13450: NAD_binding_8" amino acids 379 to 413 (35 residues), 35.8 bits, see alignment (E = 3.6e-12)

Best Hits

Swiss-Prot: 53% identical to FADH_ECOLI: 2,4-dienoyl-CoA reductase (fadH) from Escherichia coli (strain K12)

KEGG orthology group: K00219, 2,4-dienoyl-CoA reductase (NADPH2) [EC: 1.3.1.34] (inferred from 100% identity to mag:amb3454)

MetaCyc: 53% identical to 2,4-dienoyl-CoA reductase (Escherichia coli K-12 substr. MG1655)
2,4-dienoyl-CoA reductase (NADPH). [EC: 1.3.1.34]

Predicted SEED Role

"2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34)" (EC 1.3.1.34)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.1.34

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W1L7 at UniProt or InterPro

Protein Sequence (666 amino acids)

>AMB_RS17475 NADPH-dependent 2,4-dienoyl-CoA reductase (Magnetospirillum magneticum AMB-1)
MRHRHLLSPITFRGVTLPNRMIMGSMHLGFEALPDAWSRLAEFYTERARGGIGLIVTGGV
APNQQGRFGPDGTVLTSEAELPGHRLITQAVHAQGGRIIMQILHCGRYSRMKDIVAPSAI
RAPINSQAPRELSDAEIVQTIADYAECARLAVLAGYDGVEVMASEGYLINEFLALRTNKR
TDRWGGDLHGRMRFLVEIVRAVRAALGEAAMMSVRLSMVDLVEDGLAAEEVVAVARAIEA
EGCDLINSGIGWHEARVPTIAHTVPQGAWSWATARVKAAVGIPVAASNRIKTPDLAESLI
AEGQCDLVSMARPLLADPAFAAKVAAGEPDSINACIGCNQGCLDPIFSGGAATCLVNPRA
GREGEFMSRPPCETQRVAVVGGGPAGMACALDAARRGHDVTLFDAAPELGGQFILARAVP
GKEEYLATPSYYAQALKQAGVTLRLGHAATAAELLPFARIVLATGIRPRRLDLPGADHPK
VVGYEDILSGRQRAGARVAIMGSGGIGFDTALYLVGEDFEADFNAEWGIDRAFTGRGGLA
EPVAPPPPRRAVTMLQRKADRPGAGLGKTTGWIHKAHLQRHHVDMLAGVTYRFIDDAGLH
VTINGEDRLIEADTVVVCIGQEPRDDLAEELKAQGRHVHLIGGARDASRLDALAAFEEGT
RLGLAL