Protein Info for AMB_RS17325 in Magnetospirillum magneticum AMB-1

Annotation: multidrug efflux RND transporter permease subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1059 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 342 to 362 (21 residues), see Phobius details amino acids 369 to 391 (23 residues), see Phobius details amino acids 397 to 419 (23 residues), see Phobius details amino acids 440 to 461 (22 residues), see Phobius details amino acids 472 to 499 (28 residues), see Phobius details amino acids 547 to 565 (19 residues), see Phobius details amino acids 880 to 898 (19 residues), see Phobius details amino acids 905 to 927 (23 residues), see Phobius details amino acids 933 to 957 (25 residues), see Phobius details amino acids 982 to 1002 (21 residues), see Phobius details amino acids 1014 to 1036 (23 residues), see Phobius details TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 3 to 1054 (1052 residues), 1171.2 bits, see alignment E=0 PF00873: ACR_tran" amino acids 4 to 1037 (1034 residues), 1128.5 bits, see alignment E=0 PF03176: MMPL" amino acids 346 to 519 (174 residues), 39.2 bits, see alignment E=4.4e-14

Best Hits

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 65% identity to bid:Bind_0901)

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W1P6 at UniProt or InterPro

Protein Sequence (1059 amino acids)

>AMB_RS17325 multidrug efflux RND transporter permease subunit (Magnetospirillum magneticum AMB-1)
MRLSHFFIDRPIFATVVSVLITIIGAITYFALPVAQYPEIAPPTIVVSASYPGASAQVVA
DTVATPLEQQINGVENMLYMNSQATGDGNLKLTVTFALGTNLDTAQVLVQNRVAIATARL
PDDVKRIGITVAKNSPDMLMVVHVNSPDGSREQLYLSNFATLQMIDVLARLDGVGEVRIF
GARDYAMRVWIDPDKAAARNLTAGEVVKALQAQNVQIAAGVLDQPPVPKQGAFQLSVQTQ
GRLLDPAQFGEVIVKADSSGRVVRLKDVARIELGAQDYSVNGYLNGNNAVPVAVFQRPGS
NALATAERVIAKVEELSRTFPSGVSYTIAYNPTQFIAQSVKAVIKTIFEAVILVVFVVIL
FLQTWRASLIPLAAIPVSLVGTFAVLGAMGYSLNNLSLFGLVLAVGIVVDDAIVVVENVE
RNMKDGMNARSAAHQTMDEVGSALISIALVLCAVFIPAAFIPGISGQFFRQFAVTIAAST
VISLIVSLTLSPALCALLFRGHGPLDHHEETGWRRQVARFFTWFNHNFDRLAGGYGGMTR
SVVRKSGIMLLLYAGLIGAAGVEFVKTPKGFIPEMDQGYLITVVQLPPGASLSRTDAAVK
RVAAIMAETPGVAHTLAFAGLDGATFTNASNGAAIFTPLKPFEERYAQGLSANVIMADLR
KRLSTVQDAFIITIAPPPVRGIGTAGGFKMMLQDKGGHGSKALEDVALDMAAEANKIPGL
QGIFTLFNTRTPNVYADIDRVRAQMLGVPTDRVSEALQVYLGSAFVNEFNYLGRTFRVTA
QADAPFRNEVEDIANIKVRNDKGQMVPIGAVSSFRFDTGPYRVPRYNLFPAAEVQGSAAP
GYASGYALEQMRALAAERLPEGFGFEWTELALQELLAGNNGLFVFAASVLFVFLLLAAQY
ESWSLPAAVVLIVPMCLLAAVTGLIIRGLPINILAQIGFIVLIGLAAKNAILIVEFARQA
ESEGMDRIEAAVHAGRTRLRPILMTSLAFILGVLPLVAGTGAGAEMRQSLGTAVFYGMLG
VTAFGLVFTPIFYVVIRKMVAGHHARAAAAHAAEEARGR