Protein Info for AMB_RS16325 in Magnetospirillum magneticum AMB-1

Annotation: 4Fe-4S dicluster domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 618 transmembrane" amino acids 91 to 111 (21 residues), see Phobius details PF01855: POR_N" amino acids 24 to 184 (161 residues), 52.4 bits, see alignment E=1.6e-17 PF02775: TPP_enzyme_C" amino acids 379 to 501 (123 residues), 78.7 bits, see alignment E=1e-25 PF13292: DXP_synthase_N" amino acids 401 to 459 (59 residues), 21.4 bits, see alignment 3.5e-08 PF13237: Fer4_10" amino acids 548 to 603 (56 residues), 27 bits, see alignment 9.1e-10

Best Hits

KEGG orthology group: K00179, indolepyruvate ferredoxin oxidoreductase, alpha subunit [EC: 1.2.7.8] (inferred from 100% identity to mag:amb3233)

Predicted SEED Role

"Indolepyruvate oxidoreductase subunit IorA (EC 1.2.7.8)" in subsystem Aromatic amino acid interconversions with aryl acids (EC 1.2.7.8)

Isozymes

Compare fitness of predicted isozymes for: 1.2.7.8

Use Curated BLAST to search for 1.2.7.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W288 at UniProt or InterPro

Protein Sequence (618 amino acids)

>AMB_RS16325 4Fe-4S dicluster domain-containing protein (Magnetospirillum magneticum AMB-1)
MSAAVQAKPQSRILLSGNDAIARGVWEAGAKVAAAYPGTPSTEILESLSLYPDLYAEWSV
NEKVAMEVAIGGSMSGARSFCAMKHVGLNVASDAFMTVTVAGVIGGLVIAVADDVGMSSS
QNEQDSRFWGRFAHVPVLEPADSQEAYEMAKYAFELSEAYNTPVILRLTTRVCHVKAVVQ
VGEKVAHDIGGFKSDARRWVMTPANAKGLLPAQIAREGKLQALAEASPMNFLDDGPDKRV
GFVTSGPAYMHVRESFPDAPVLKLGFTYPVPVALVEKLAAQVEHLVVVEETEPLMEQELK
AAGLQAIHGKDLLPRLGELTPNVLIPAIKTFLGEPLPPTTTYPKFDPFPRPPTMCAACPH
MGVYYSLARMRKNVQISGDIGCYTLGAGHPWNALDSTISMGASMGIALGMDKARTEETKD
KAVVAVIGDSTFLHMGMQGLLDIVYNRGNVTVLILDNRTTGMTGGQNHAGTGKGLKGDDT
PRVDFAKLVEALGVKPERISKIDPYVLPVVFKTIKQETAIPEVSVIITDRPCVLSEFYTK
IEPYKVIDEDCTGCGNCINVGCPAITVKRSESKVRPDGKVNELKFTTIDTAMCTGCHMCV
ESCGPKAIVLAKPAAAAE