Protein Info for AMB_RS16130 in Magnetospirillum magneticum AMB-1

Annotation: phosphoglycerate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 526 TIGR01327: phosphoglycerate dehydrogenase" amino acids 3 to 523 (521 residues), 695.6 bits, see alignment E=2.4e-213 PF00389: 2-Hacid_dh" amino acids 4 to 312 (309 residues), 129.7 bits, see alignment E=1.2e-41 PF02826: 2-Hacid_dh_C" amino acids 106 to 280 (175 residues), 214.6 bits, see alignment E=1.4e-67 PF19304: PGDH_inter" amino acids 323 to 441 (119 residues), 161.8 bits, see alignment E=1.4e-51 PF01842: ACT" amino acids 454 to 515 (62 residues), 32.4 bits, see alignment E=1.3e-11

Best Hits

Swiss-Prot: 44% identical to SERA_BACSU: D-3-phosphoglycerate dehydrogenase (serA) from Bacillus subtilis (strain 168)

KEGG orthology group: K00058, D-3-phosphoglycerate dehydrogenase [EC: 1.1.1.95] (inferred from 100% identity to mag:amb3193)

Predicted SEED Role

"D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95)" in subsystem Glycine and Serine Utilization or Pyridoxin (Vitamin B6) Biosynthesis or Serine Biosynthesis (EC 1.1.1.95)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.95

Use Curated BLAST to search for 1.1.1.95

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W2C8 at UniProt or InterPro

Protein Sequence (526 amino acids)

>AMB_RS16130 phosphoglycerate dehydrogenase (Magnetospirillum magneticum AMB-1)
MPKVLISDKLSPAAVQIFKDRGIETDVKTGLAPDELKAIIGQYDGLAIRSNTKVTTEILA
AATNLKVVGRAGIGVDNVDVPAATARGIVVMNTPFGNSITTAEHAIAMMFALAREIPEAN
ASTHAGKWEKNRFMGVELTGKVLGIVGCGNIGAIVADRAQGLRMRVIAYDPFLSVERAKE
LNVEKVELDELFPRADFITLHTPLTDATRNIIDAKAMAKMKKGVRIINCARGGLVVEEDL
LAALESGQVAGAALDVFKTEPAKENALFGNPKVVCTPHLGASTSEAQENVALQVAEQMAD
YLLTGAVTNALNIPSVSAEDAPKLRPYMTLANQIGSFAGQLTETGIKDVKIEYLGHVASL
NTKPLTAMVLEGLLKPMNEAVNMVNAPVVAKERNIKVSEVKSESEGDYHTLVRLTVTTDK
RERSVAGTLFGGNKARVVEIKGISIEAELGQNMLYVTNQDKPGFIGALGSLLGANGVNIA
TFHLGRSEAGGDAILLTQVDQPVSNDLLEKVRALPQVVQAKFLTFA