Protein Info for AMB_RS16035 in Magnetospirillum magneticum AMB-1

Annotation: L(+)-tartrate dehydratase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 332 TIGR00722: hydrolyase, tartrate alpha subunit/fumarate domain protein, Fe-S type" amino acids 43 to 314 (272 residues), 321 bits, see alignment E=2.8e-100 PF05681: Fumerase" amino acids 46 to 314 (269 residues), 261.4 bits, see alignment E=4.6e-82

Best Hits

Swiss-Prot: 62% identical to TTDA_ECOUT: L(+)-tartrate dehydratase subunit alpha (ttdA) from Escherichia coli (strain UTI89 / UPEC)

KEGG orthology group: K03779, L(+)-tartrate dehydratase alpha subunit [EC: 4.2.1.32] (inferred from 100% identity to mag:amb3179)

MetaCyc: 61% identical to L(+)-tartrate dehydratase subunit alpha (Escherichia coli K-12 substr. MG1655)
L(+)-tartrate dehydratase. [EC: 4.2.1.32]

Predicted SEED Role

"L(+)-tartrate dehydratase alpha subunit (EC 4.2.1.32)" (EC 4.2.1.32)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.32

Use Curated BLAST to search for 4.2.1.32

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W2E2 at UniProt or InterPro

Protein Sequence (332 amino acids)

>AMB_RS16035 L(+)-tartrate dehydratase subunit alpha (Magnetospirillum magneticum AMB-1)
MRDLSPPDDKTTITSKIKRGCRAKYLKCSRRLSVTSQAPVEVMTDIMTKFTSYIGKRLPT
DIKEKLADLREAETNPMANVLFDSMAENQEAADKLNRPSCQDTGVIQYFITAGAKFPLLG
ELEDILRNATLGATKLGPLRHNAVEAFDEKNTGTNTGSRIPWLDWEIVPNDDSVTIDVYM
AGGGCTLPGASTVLMPGQGYEGVVDFVFDVVTSRGVNACPPLLIGVGVSTSAETAARLSK
KAILRPVGSQHGNANAAKMETLLEDGLNELGIGPQGLTGNSSVMGVNIESSARHPSTIGV
AVSTGCWAHRRGRIRFNADLSYEIVSHEGVVL