Protein Info for AMB_RS15430 in Magnetospirillum magneticum AMB-1

Annotation: 1,4-alpha-glucan branching protein GlgB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 730 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 107 to 726 (620 residues), 947.4 bits, see alignment E=1.9e-289 PF02922: CBM_48" amino acids 122 to 206 (85 residues), 79.2 bits, see alignment E=3.6e-26 PF00128: Alpha-amylase" amino acids 278 to 345 (68 residues), 31.1 bits, see alignment E=2.9e-11 PF02806: Alpha-amylase_C" amino acids 630 to 728 (99 residues), 75.7 bits, see alignment E=4.3e-25

Best Hits

Swiss-Prot: 100% identical to GLGB_MAGSA: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 100% identity to mag:amb3065)

MetaCyc: 54% identical to 1,4-alpha-glucan branching enzyme (Mycobacterium tuberculosis H37Rv)
1,4-alpha-glucan branching enzyme. [EC: 2.4.1.18]

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W2Q6 at UniProt or InterPro

Protein Sequence (730 amino acids)

>AMB_RS15430 1,4-alpha-glucan branching protein GlgB (Magnetospirillum magneticum AMB-1)
MPEAAKLIRSSDADAIARGRHGDPFAVLGPHAVKGGTVIRTFQPQARTVFVLAADGETEM
SRAHPDGLFAVKLKGSPAYRLRAVRHDGGTEELDDPYRFPPVLGDLDVHLLAEGTHLRTF
EKLGAQVRIVDGVAGVDFALWAPNASRVSVVGDFNGWDGRRHPMRLRHEAGMWEIFIPGL
GQGAVYKYEIVASDGRLLPLKADPYGHFAEVPPKTASVVWELGRRDWADADWMAAQKARN
DRHAPISIYEVHLGSWRRVPEDCNRPLSYLEMADQLGDYVADLGFTHVEFLPIHEHPFGG
SWGYQPVGLFAPTSRYGTPDEFRTLVDRLHQKGIGVIIDWVAGHFPNDPHGLHHFDGTHL
YEHEDPRLGVHKDWNTLIYNYGRSEVVNYLYANALYWLEQYHVDGLRVDAVASMLYLDYS
REPGEWIPNRHGGNENLEAIDFLRRMNMLVYAEHPGAMTIAEESTAWPMVSRPVHLGGLG
FGYKWNMGWMHDTLRYFSKDPIHRRYHHDSLTFAQLYAYHENFVLPLSHDEVVHGKGSIF
GRMPGDPWQRFANLRAYYGFMWTQPGKKLLFMGQEFAQQSEWNEDASLDWHLLGDGRNEG
VMRLIRDLNRLYRTEPALHQLDNEPAGFAWIDCNDRDNSVLTWLRKGFDPGDFLVVAGNY
TPMVRDSYRIGVPEPGWYRELLNTDSEWYGGANIHNGGGVHTEEVPWHGHGFSICLRLPP
LATCVFKRER