Protein Info for AMB_RS15255 in Magnetospirillum magneticum AMB-1
Annotation: IscS subfamily cysteine desulfurase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 66% identical to ISCS_RICRO: Cysteine desulfurase IscS (iscS) from Rickettsia rickettsii (strain Iowa)
KEGG orthology group: K04487, cysteine desulfurase [EC: 2.8.1.7] (inferred from 100% identity to mag:amb3028)MetaCyc: 62% identical to mitochondrial cysteine desulfurase NFS1 (Homo sapiens)
Cysteine desulfurase. [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]
Predicted SEED Role
"Cysteine desulfurase (EC 2.8.1.7), IscS subfamily" in subsystem Alanine biosynthesis or Thiamin biosynthesis (EC 2.8.1.7)
MetaCyc Pathways
- superpathway of L-alanine biosynthesis (4/4 steps found)
- L-alanine biosynthesis III (1/1 steps found)
- L-cysteine degradation IV (1/1 steps found)
- cytidylyl molybdenum cofactor sulfurylation (1/2 steps found)
- bis(guanylyl molybdopterin) cofactor sulfurylation (1/3 steps found)
- tRNA-uridine 2-thiolation (mammalian mitochondria) (1/4 steps found)
- tRNA-uridine 2-thiolation (yeast mitochondria) (1/4 steps found)
- superpathway of thiamine diphosphate biosynthesis II (6/11 steps found)
- thiazole component of thiamine diphosphate biosynthesis II (3/7 steps found)
- superpathway of thiamine diphosphate biosynthesis I (5/10 steps found)
- molybdopterin biosynthesis (2/6 steps found)
- thiazole component of thiamine diphosphate biosynthesis I (2/6 steps found)
- tRNA-uridine 2-thiolation (thermophilic bacteria) (1/5 steps found)
- [2Fe-2S] iron-sulfur cluster biosynthesis (3/10 steps found)
- tRNA-uridine 2-thiolation (cytoplasmic) (1/8 steps found)
- tRNA-uridine 2-thiolation and selenation (bacteria) (2/11 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.8.1.7
Use Curated BLAST to search for 2.8.1.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q2W2U3 at UniProt or InterPro
Protein Sequence (418 amino acids)
>AMB_RS15255 IscS subfamily cysteine desulfurase (Magnetospirillum magneticum AMB-1) MTALGKTTRPSLPRAPGAPVYLDYQATTPCDPRVVEVMLPWFTEKFGNPHSRNHRYGWEA EEAVEKAREQIADIIGADSKEVIFTSGATESNNLAIKGVAHFYKEKKNHIVTVVTEHKCV LDTCRYLEQEGFAVTYLPVKADGLIDLAELEAAITDQTVIVSVMAVNNEIGVIQPLAEIG ALCRKKGAFFHTDCAQAVGKIPLDVNAMNIDLMSISGHKLYGPKGIGALYVRRRPRVRLV PLITGGGQERGMRSGTLATPLCVGFGEACAIAKAEMGAENERLMGLRNRLLGGLRQRLPE IYVNGDLDHRIPGNLNISFAFVEGEGLMMGVKELAVSSGSACTSASLEPSYVLRALGVDV EMAHTSLRFGLGRFTTDEDIEFAIDHITEAVEHLRAMSPLWDMHLEGVDLKSIEWAEH