Protein Info for AMB_RS15110 in Magnetospirillum magneticum AMB-1

Annotation: error-prone DNA polymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1022 TIGR00594: DNA polymerase III, alpha subunit" amino acids 5 to 810 (806 residues), 520.9 bits, see alignment E=3.9e-160 PF02811: PHP" amino acids 7 to 122 (116 residues), 39.5 bits, see alignment E=1.9e-13 PF07733: DNA_pol3_alpha" amino acids 269 to 523 (255 residues), 293.8 bits, see alignment E=3.5e-91 PF17657: DNA_pol3_finger" amino acids 526 to 691 (166 residues), 189.2 bits, see alignment E=8.8e-60 PF14579: HHH_6" amino acids 765 to 849 (85 residues), 66.6 bits, see alignment E=4.7e-22 PF01336: tRNA_anti-codon" amino acids 928 to 1003 (76 residues), 43.5 bits, see alignment 6.4e-15

Best Hits

Swiss-Prot: 59% identical to DNE22_AGRFC: Error-prone DNA polymerase 2 (dnaE2-2) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K14162, error-prone DNA polymerase [EC: 2.7.7.7] (inferred from 100% identity to mag:amb3000)

Predicted SEED Role

"Error-prone repair homolog of DNA polymerase III alpha subunit (EC 2.7.7.7)" (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W2X1 at UniProt or InterPro

Protein Sequence (1022 amino acids)

>AMB_RS15110 error-prone DNA polymerase (Magnetospirillum magneticum AMB-1)
MTPYAELQTLSNYTFLEGASHADELAITASALGLEAIAICDRNTLSGIVRAHVAAKAAGI
RLVVGARLDLTDGASLLCFPTDRAAYGRLSRLLTLGRRRAPKGECHLARADVAEHAEGQI
LVLIAPDPLEEGFAAQLAEWRTVVGDRLYLAATRRFRGDDGERLRVLAGFGIPLVATNDV
LYHTPARRPLADVLTCIRQGTTLAAAGWALSAHGERHLKSPAEMARLFAACPEALANGLE
VVRRCRFSLDELAYEYPNEVAAGMDPQERLEILTRQGAARRYPRGVPPKVQAQLAHELAL
IRQLGFAPYFLTVHAIVAFAESRGILCQGRGSAANSAVCYCLRVTPVDPAQMDLLFERFI
SAERGEPPDIDVDFEHERREEVIQHIYDTYGRHRAGLTATVIHYRTRSAIRDVGKVMGLS
EDTVEALAKANSGWGRRGIKDEHVRELGLDPTEPGLARTLSLAEELTGFPRHLSQHVGGF
VISKGRLDELVPIENAGMEDRTVIQWDKDDLDALGLMKVDVLALGMLSCIRKAFDLLRLH
HGRDLCLATLPREDASTYDMLCKADAVGVFQVESRAQMSMLPRLRPRRFYDLVVEVAIVR
PGPIQGGMVHPYLRRRQGKETVDYPSEELRQVLGKTLGVPLFQEQAMKIAMVAAGFTASE
ADGLRRAMATFRHTGQVGAYGDKLIGGMVARGYERDFAERVFKQIEGFGEYGFPESHAAA
FAHLVYVSAWLKCHYPAAFACALLNSQPMGFYAPAQIVRDAADHGVEIRPVDVNHSDWDC
TLEGKALRLGLRQVSGLARRDGERLAAARGAGYASAYGLWRTSGLERAALDRLAAADAFG
SMGLGRREAAWAIKALDAPPLPLFEEAPPPPEAKVALPPASVGEEVVGDYAALKLSLKCH
PLAVLRDPFRVLGIMTNASLKDRKGGRVAVSGLVLVRQRPGSANGVLFITLEDETGIANI
VVWPDMFETFRRPILGGHLLRVDGRVQSEDGVIHVVAEKIEDLSAHLGALARGSHYPSRD
FR