Protein Info for AMB_RS14650 in Magnetospirillum magneticum AMB-1

Annotation: cadmium-translocating P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 804 transmembrane" amino acids 187 to 203 (17 residues), see Phobius details amino acids 209 to 230 (22 residues), see Phobius details amino acids 242 to 264 (23 residues), see Phobius details amino acids 270 to 288 (19 residues), see Phobius details amino acids 423 to 443 (21 residues), see Phobius details amino acids 455 to 478 (24 residues), see Phobius details amino acids 757 to 774 (18 residues), see Phobius details amino acids 780 to 798 (19 residues), see Phobius details PF00403: HMA" amino acids 102 to 159 (58 residues), 45 bits, see alignment 2.3e-15 TIGR01511: copper-translocating P-type ATPase" amino acids 225 to 801 (577 residues), 577.2 bits, see alignment E=8.3e-177 TIGR01525: heavy metal translocating P-type ATPase" amino acids 244 to 800 (557 residues), 605.2 bits, see alignment E=3e-185 TIGR01512: cadmium-translocating P-type ATPase" amino acids 261 to 801 (541 residues), 407.6 bits, see alignment E=1.5e-125 TIGR01494: HAD ATPase, P-type, family IC" amino acids 278 to 788 (511 residues), 281.1 bits, see alignment E=2.7e-87 PF00122: E1-E2_ATPase" amino acids 306 to 485 (180 residues), 186.6 bits, see alignment E=6.3e-59 PF00702: Hydrolase" amino acids 503 to 712 (210 residues), 110.1 bits, see alignment E=3.8e-35

Best Hits

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 100% identity to mag:amb2911)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W360 at UniProt or InterPro

Protein Sequence (804 amino acids)

>AMB_RS14650 cadmium-translocating P-type ATPase (Magnetospirillum magneticum AMB-1)
MEAIQVVHQLRRRVRVVVPALKGEAERLCLLEILLRKHAAIGEVRVVPALASLAVRFDPR
RMPAENLLRLLDTVAGNVARAPRQKPPPPALGDDSQQAEVSVAVEGMTCASCAALIQMSL
NRDPAIRSANVNYATATATVVGTLDRPALEARIGALGYEARPMDTLSQRRMIVERERRHV
QDAKRRAVVACLLSLPVMVIGMAMPRSRFWHVVEFALTTPVVLGAGRPFFSRAAKLAKSR
AANMDTLIALGSGAAYGHSVASLLAGRHHLYFEAAAGIVSFVLLGRWLEERAKGKAGDAI
RRLLDLQPPTATLVRDEVEVVVPVDDLVVGDILVVRPGERVPVDGTVTGGRTTLDESMVT
GESMPVVKGPGDGVIGGCINGAGSFRMRATAVGQDTVLAGIVRMVDHAQAAKLPVQRLAD
RVSSVFVPGVVAVAGVTFATWLAGGARVSTALGNAVSVLLIACPCALGLATPTAIMAATG
QAARRGIYIRNGEALETASKLGVLVFDKTGTVTEGRPVVSHFSARPGFDPDQVLALVAAA
EAGSEHHLGRSVVDYARSRGIEPSSAEEFMAEIGRGIRARVGRHVVQVGSAAFLAEEGVG
VDQLPSLDGQTPVLAAIDGKFAALFAISDRPRPTSAAAIARLHEMGIRTIMVTGDVEAAA
RHIAAEVGIPEVVAQASPSRKQEIVAELRAAGEAVGMIGDGINDAPALAAADVGFAIGTG
TDVAIEAAPVTLVNGDIAKVAEMIELSRKTMRIIRQNLVWAMGYNTIAIPGAALGELSPM
VASSAMALSSVSVVTNSLRLQKDR