Protein Info for AMB_RS14495 in Magnetospirillum magneticum AMB-1

Annotation: aminotransferase class V-fold PLP-dependent enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 43% identical to CSD_BACHD: Probable cysteine desulfurase (csd) from Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)

KEGG orthology group: None (inferred from 100% identity to mag:amb2879)

MetaCyc: 38% identical to L-cysteine desulfurase (Escherichia coli K-12 substr. MG1655)
Selenocysteine lyase. [EC: 4.4.1.16]; Cysteine desulfurase. [EC: 4.4.1.16, 2.8.1.7]

Predicted SEED Role

"Cysteine desulfurase (EC 2.8.1.7)" in subsystem Alanine biosynthesis (EC 2.8.1.7)

MetaCyc Pathways

superpathway of L-alanine biosynthesis (4/4 steps found)
L-alanine biosynthesis III (1/1 steps found)
L-cysteine degradation IV (1/1 steps found)
cytidylyl molybdenum cofactor sulfurylation (1/2 steps found)
bis(guanylyl molybdopterin) cofactor sulfurylation (1/3 steps found)
tRNA-uridine 2-thiolation (mammalian mitochondria) (1/4 steps found)
tRNA-uridine 2-thiolation (yeast mitochondria) (1/4 steps found)
superpathway of thiamine diphosphate biosynthesis II (6/11 steps found)
thiazole component of thiamine diphosphate biosynthesis II (3/7 steps found)
superpathway of thiamine diphosphate biosynthesis I (5/10 steps found)
molybdopterin biosynthesis (2/6 steps found)
thiazole component of thiamine diphosphate biosynthesis I (2/6 steps found)
tRNA-uridine 2-thiolation (thermophilic bacteria) (1/5 steps found)
[2Fe-2S] iron-sulfur cluster biosynthesis (3/10 steps found)
tRNA-uridine 2-thiolation (cytoplasmic) (1/8 steps found)
tRNA-uridine 2-thiolation and selenation (bacteria) (2/11 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.7, 4.4.1.16

Use Curated BLAST to search for 2.8.1.7 or 4.4.1.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W392 at UniProt or InterPro

Protein Sequence (400 amino acids)

>AMB_RS14495 aminotransferase class V-fold PLP-dependent enzyme (Magnetospirillum magneticum AMB-1)
MSQSMREAFPALSRPGFHYLDNAAMAQLPGLVSEAVASFNRVARSNVRRGLHESAQRADA
AYDGAREAVGRFLNAPAEEVVFTGGCTLALNLAAWGLGRTLGPGDEVVVSRLEHHSACLP
WMALAKERGFTLRWLPVTPEGRLDLGDPGAVISSRCKVVAVTHASNVTGAISDLPRLAEA
AHAVGARLVVDGAQMVPHGRVDPRALGADLYAFAGHKAYGPTGVGVLWGRAEVLDGMAPL
VMGGGMVGAVDDDGFTWGEVPHRFEAGTPPVAQAVGLAAALDWMSGLDWQAVHQQESALT
WHLLDGLRAMEGVRIVGPLGLEARVPVVSFTVDGCHPHDLAHLLAERGVAVRGGAHCARP
LMSVLGLEEGTVRASLAPYSDRSDVEALLQGLAEAVKVLR