Protein Info for AMB_RS14385 in Magnetospirillum magneticum AMB-1

Annotation: thiamine phosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00693: thiamine-phosphate diphosphorylase
  • amino acids 7 to 200 (194 residues), 164.7 bits, E=8e-53
• PF02581: TMP-TENI
  • amino acids 8 to 188 (181 residues), 178.1 bits, E=5.6e-57

Best Hits

Swiss-Prot: 66% identical to THIE_RHOCS: Thiamine-phosphate synthase (thiE) from Rhodospirillum centenum (strain ATCC 51521 / SW)

KEGG orthology group: K00788, thiamine-phosphate pyrophosphorylase [EC: 2.5.1.3] (inferred from 66% identity to rru:Rru_A1080)

Predicted SEED Role

"Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3)" in subsystem Thiamin biosynthesis (EC 2.5.1.3)

MetaCyc Pathways

• thiamine diphosphate biosynthesis I (E. coli) (1/2 steps found)
• thiamine diphosphate biosynthesis II (Bacillus) (1/2 steps found)
• thiamine diphosphate salvage II (3/5 steps found)
• thiamine diphosphate biosynthesis III (Staphylococcus) (1/3 steps found)
• thiamine diphosphate biosynthesis IV (eukaryotes) (1/3 steps found)
• thiamine diphosphate salvage V (1/3 steps found)
• superpathway of thiamine diphosphate biosynthesis II (6/11 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)
• thiamine diphosphate salvage IV (yeast) (3/7 steps found)
• superpathway of thiamine diphosphate biosynthesis I (5/10 steps found)
• thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (3/8 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.3

Use Curated BLAST to search for 2.5.1.3

Protein Sequence (212 amino acids)

>AMB_RS14385 thiamine phosphate synthase (Magnetospirillum magneticum AMB-1)
MSRDSCRLYLITPPVIEKPFEWVSTLEAALDAGDVACLQIRLKGLEDDALARIVDVLRPP
AQRRGVAVLLNDRPDLAFETGCDGVHVGQTDASYKAARQAVGPEGIVGITCHDSRHLAME
AGEAGADYVAFGAFFPTETKEAPTRAEIELLEWWHGLFTVPCVAIGGITVENCRPLVTAG
ADFLAVSGGVWNHPEGPEAAVRAFAKICADGG