Protein Info for AMB_RS14295 in Magnetospirillum magneticum AMB-1

Annotation: RNA degradosome polyphosphate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 712 TIGR03705: polyphosphate kinase 1" amino acids 19 to 690 (672 residues), 945.2 bits, see alignment E=1.1e-288 PF13089: PP_kinase_N" amino acids 20 to 124 (105 residues), 128.6 bits, see alignment E=2.9e-41 PF02503: PP_kinase" amino acids 135 to 307 (173 residues), 168.8 bits, see alignment E=2.7e-53 PF17941: PP_kinase_C_1" amino acids 337 to 501 (165 residues), 246.1 bits, see alignment E=3.5e-77 PF13090: PP_kinase_C" amino acids 508 to 677 (170 residues), 199.4 bits, see alignment E=8.1e-63

Best Hits

Swiss-Prot: 65% identical to PPK1_ACICJ: Polyphosphate kinase (ppk) from Acidiphilium cryptum (strain JF-5)

KEGG orthology group: K00937, polyphosphate kinase [EC: 2.7.4.1] (inferred from 100% identity to mag:amb2840)

Predicted SEED Role

"Polyphosphate kinase (EC 2.7.4.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism or Purine conversions (EC 2.7.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.4.1

Use Curated BLAST to search for 2.7.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W3D1 at UniProt or InterPro

Protein Sequence (712 amino acids)

>AMB_RS14295 RNA degradosome polyphosphate kinase (Magnetospirillum magneticum AMB-1)
MTHSQAVDIPAIDIESKDRFINRELSWLAFNGRVIEEAFNPHHPLLERLRFLSISAANLD
EFYMVRVAGLKGQVDAGVMTTSQDGLTPAQQLAAINSEAARLLEAQKKCWRELKVELRGA
GIAVIDRSELSKADMKWLEGRFMEHVFPVLTPLAIDPAHPFPFLPNGSSALVLHLNKLDD
GEVLRALLPLPGQIERFTRLPGDGIRFLPLEEFVLLFLDQLFPGFRMESFGHFRVIRDTE
MDIDEEAEDLVRVFESALKRRRRGHVIRLTFSAGIPADLKDLAIAEMHADIEDVFEMDGL
LGLVDTKRLIVDERPDLLFPPYHARFPERIRDFGGDCFAAIRKKDIVVHHPFESFDVVVQ
FIRQAAADPAVVAIKQTLYRTSADSPIVKALVEAAEAGKSVTAMVELKARFDEEANIRWA
RDLERAGAQVVFGFLELKTHAKISLVVRREGGTLVSYVHFGTGNYHPITAKVYTDLSFFT
CDPALCRDAAKTFNFMTGYAKPDGMESLAIAPIYLRKKVLSLIEDEIANVKDGKPGAIWA
KMNSLVCPQLIDALYRASQAGVKVDLVIRGICCLRPGVPGLSDNIRVKSIVGRFLEHSRV
ICFGNGHRMPSRHAKIFISSADWMPRNMDWRVESFVPIENPTVHRQILDQIMVANLKDQA
QTWILRPDGVYERFQAEEGAFNAHTYFMTNPSLSGRGSAGERGRKSKNLQLA