Protein Info for AMB_RS14100 in Magnetospirillum magneticum AMB-1
Annotation: glutamate--tRNA ligase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to SYE2_MAGSA: Glutamate--tRNA ligase 2 (gltX2) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)
KEGG orthology group: K01885, glutamyl-tRNA synthetase [EC: 6.1.1.17] (inferred from 100% identity to mag:amb2803)Predicted SEED Role
"Glutamyl-tRNA synthetase (EC 6.1.1.17)" in subsystem Heme and Siroheme Biosynthesis or tRNA aminoacylation, Glu and Gln (EC 6.1.1.17)
MetaCyc Pathways
- adenosylcobalamin biosynthesis II (aerobic) (28/33 steps found)
- adenosylcobalamin biosynthesis I (anaerobic) (29/36 steps found)
- tRNA charging (18/21 steps found)
- tetrapyrrole biosynthesis I (from glutamate) (5/6 steps found)
- superpathway of heme b biosynthesis from glutamate (7/10 steps found)
- (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.1.1.17
Use Curated BLAST to search for 6.1.1.17
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q2W3G8 at UniProt or InterPro
Protein Sequence (477 amino acids)
>AMB_RS14100 glutamate--tRNA ligase (Magnetospirillum magneticum AMB-1) MTVVTRFAPSPTGFLHIGGGRTALFNWLFARHHGGKFLLRIEDTDRARSTDAAVEAIFDG IKWLGLDWDGEAVMQFARACRHAEVARQLLDEGKAYRCYCTQDELTAIREEQKAKGQPMR YPGIWRDRPATDAPEGAPFVVRLKAPAEGETIIADLVQGDVRVANDQLDDMVLLRADGTP TYMLSVVVDDHDMGITHVIRGDDHLTNAFRQYQLYKACGWEVPTFAHIPLIHGPDGAKLS KRHGALGVDAYRDMGFLPEAMRNYLLRLGWSHGDDEIISTEQAVEWFTLDSVGRSPSRFD FVKLTNLNGHYMRGADDGRLTEVLVPLLEAKTGKALSGESVARLRTGMTGLKERAKTMVE LADSALFYVAERPLALDEKAAKTMADETATADLAAYRTEVKELGAWTRDTLEDAARRLAE ARGQKLGKIAQPLRAALAGSSVSPPIFEVMEVLGREESLGRIEDALGGTRPTTSTAA