Protein Info for AMB_RS14080 in Magnetospirillum magneticum AMB-1

Annotation: D-amino-acid dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 422 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details PF02737: 3HCDH_N" amino acids 2 to 35 (34 residues), 22.3 bits, see alignment 6.1e-08 PF00070: Pyr_redox" amino acids 2 to 33 (32 residues), 24.7 bits, see alignment (E = 1.5e-08) PF01266: DAO" amino acids 2 to 399 (398 residues), 255.8 bits, see alignment E=5.1e-79 PF00890: FAD_binding_2" amino acids 3 to 77 (75 residues), 22.8 bits, see alignment E=2.7e-08 PF02558: ApbA" amino acids 3 to 48 (46 residues), 27.6 bits, see alignment 1.1e-09 PF13450: NAD_binding_8" amino acids 5 to 38 (34 residues), 30.6 bits, see alignment 1.9e-10

Best Hits

Swiss-Prot: 100% identical to DADA_MAGSA: D-amino acid dehydrogenase (dadA) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K00285, D-amino-acid dehydrogenase [EC: 1.4.99.1] (inferred from 100% identity to mag:amb2799)

Predicted SEED Role

"D-amino acid dehydrogenase small subunit (EC 1.4.99.1)" in subsystem Pyruvate Alanine Serine Interconversions or Respiratory dehydrogenases 1 (EC 1.4.99.1)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W3H2 at UniProt or InterPro

Protein Sequence (422 amino acids)

>AMB_RS14080 D-amino-acid dehydrogenase (Magnetospirillum magneticum AMB-1)
MKVVVIGAGVVGTASAWYLAKAGHEVTVVDRREGAGLETSFANGGQISPCHAEPWANPSV
LPKVLKWLGREDAPLLFRWNRWDPALWAWGLRFLANCSRSRAEINTERTLRVALYSRACL
GELRAETGIAYDQQVRGILHVYRDGAEFEHACRAAEVMIRHGLRRLPRTPAECTAIEPAL
GAVQGELAGGIYTPDDESGDAHKFTRELAALAAAKGVEFRWNVPIQSLLADGDRVAGLAT
SDGTIRAESYVLAAGCDSPLLARPLGLRLPIIPAKGYSVTVPVDNHAGAPLVSITDDEHK
MVYSRLGDRLRAAGTAEMAGYDRMPNPVRNRLILDNARRLFPDGGDFDRAEPWAGLRPVT
PDSVPLLGATPLRNLWLNTGHGTLGWTMSCGSGRIVADLVSGRPSAISMDGLGIDRFTSY
LW