Protein Info for AMB_RS13875 in Magnetospirillum magneticum AMB-1

Annotation: 8-amino-7-oxononanoate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 392 TIGR00858: 8-amino-7-oxononanoate synthase" amino acids 21 to 384 (364 residues), 429.1 bits, see alignment E=5.7e-133 PF00155: Aminotran_1_2" amino acids 41 to 384 (344 residues), 171.7 bits, see alignment E=1.3e-54

Best Hits

Swiss-Prot: 100% identical to BIOF_MAGSA: Putative 8-amino-7-oxononanoate synthase (bioF) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K00652, 8-amino-7-oxononanoate synthase [EC: 2.3.1.47] (inferred from 100% identity to mag:amb2759)

MetaCyc: 38% identical to 8-amino-7-oxononanoate synthase (pimeloyl-CoA-dependent) (Bacillus subtilis subtilis 168)
8-amino-7-oxononanoate synthase. [EC: 2.3.1.47]; 2.3.1.47 [EC: 2.3.1.47]

Predicted SEED Role

"8-amino-7-oxononanoate synthase (EC 2.3.1.47)" in subsystem Biotin biosynthesis (EC 2.3.1.47)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.47

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W3L2 at UniProt or InterPro

Protein Sequence (392 amino acids)

>AMB_RS13875 8-amino-7-oxononanoate synthase (Magnetospirillum magneticum AMB-1)
MKGLDDSIGRFLDGLDQAGRKRVLRPVDPLPAGRVRVGGRELVNFSSNDYLGLSRHPEVV
ERSRRWLNEYGAGSGASRLVTGHLAAMEALEAKIARCKQTEAALILASGWQCNASVLPAL
LDKALWGAEPLVFADKLIHASLHAGLELSGARRYRYRHDDLDHLESLLKAHADKEGPRFI
VTETVFSMDGDVTDMAALAALASRWDAFLYVDEAHATGVLGANGFGLSPGMGAELAMGTF
SKGLGSFGAYVACSARLRHYLINRASGLIYATGLPPAVLGAIDAALDLVPRLEGERTRLQ
MMGRRLRDGLRAAGLDTGPSASQIVPLILGDEGRTLAVAKALEDRGILGIAIRPPTVPPG
TSRIRFALSAVHSDADLDRLLAAILDAVEATP