Protein Info for AMB_RS13810 in Magnetospirillum magneticum AMB-1

Annotation: ribonucleoside-diphosphate reductase, adenosylcobalamin-dependent

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1223 PF08471: Ribonuc_red_2_N" amino acids 31 to 147 (117 residues), 140.9 bits, see alignment E=1.3e-45 TIGR02504: ribonucleoside-diphosphate reductase, adenosylcobalamin-dependent" amino acids 130 to 754 (625 residues), 482 bits, see alignment E=1.4e-148 PF02867: Ribonuc_red_lgC" amino acids 198 to 754 (557 residues), 359.6 bits, see alignment E=2.9e-111 amino acids 807 to 913 (107 residues), 56.9 bits, see alignment E=1.7e-19

Best Hits

KEGG orthology group: K00525, ribonucleoside-diphosphate reductase alpha chain [EC: 1.17.4.1] (inferred from 72% identity to azl:AZL_002950)

Predicted SEED Role

"Ribonucleotide reductase of class II (coenzyme B12-dependent) (EC 1.17.4.1)" in subsystem Ribonucleotide reduction (EC 1.17.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.17.4.1

Use Curated BLAST to search for 1.17.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W3M5 at UniProt or InterPro

Protein Sequence (1223 amino acids)

>AMB_RS13810 ribonucleoside-diphosphate reductase, adenosylcobalamin-dependent (Magnetospirillum magneticum AMB-1)
MRVQRHYTKSGETAYDGIQFRKATSEIRNPDGSVVFSAADIEVPADWSQVACDVLAQKYF
RKAGVPAKLKRVAEKGVPDWLCRHEPDSEALSKLPEKERIVGEKSAKQVFDRLAGTWTYW
GWKGGYFNAEDDALAFRDEMACMLARQMGAPNSPQWFNTGLHWAYGIDGPGQGHSYVDFK
TGKLVRSKSAYEHPQPHACFIQSIEDDLVNEGGIMDLWVREARLFKYGSGTGTNFSRLRG
EGEKLSGGGRSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVVVDVDHPDIEKFISWKVRE
EQKVAALVAGSRLAARHLTEVMAACREWDGQDGEARFDPKINTRLKKAIIAGRKSEIPEN
YIQRVIQFARQGYTQIDFPVLDTDWDSEAYLTVSGQNSNNSVRVDNGFLNAVLHDADWNL
KHRNSAKAAKTLKARDLWEQIGEAAWACADPGIQFDTTINEWHTCPESGRINASNPCSEY
MFLDDTACNLASLNLLCFRNEDGSFDVDGFRHACRLWTMVLEISVLMAQFPSPKIAELSY
EFRTLGLGYANVGGLLMASGIPYDSDKGRALTGAITALMTGEAYATSADMAEELGAFEGF
EKNRAAMMRVIRNHRRAAHGMGTGFEGLSVTPVPLDAANCPDAPLLAASRQAWDEALAKG
EKHGFRNAQATVVAPTGTIGLVMDCDTTGIEPDFALVKFKKLAGGGYFKIINRMVPEALR
TLGYNEGDIAEIIRYAVGHGSLRGAPHVNHDTLKAKGFDDAMLDKIESQLESAFDIKFVF
NKYGIGEQFCTETLGIPKDKLDDYTFDMLAWLGFTREQIDAANTYVTGAMTLEGAPFLKD
EHLPVFDCASPCGRVGKRYLSVDSHIRMMAAAQPFISGAISKTINMANNATVEDCKNAYM
LSWRLGIKANALYRDGSKLSQPLQASLLEDAGELADELADASATSRTEIVAERMVERMIQ
QVQARRKLPDRRKGYIQKAIVGGHKVYLHTGEYEDGKLGEIFIDMHKEGAAFRAMMNNFA
IAISVGLQYGVPLEEFVEAFTFTRFEPSGMVEGNDTIKMSSSILDYIFRELAISYLGRND
LAHVEPADLTPDTVGRGAQEGLPEGAVAAAAAQQLAVVEKVASRGYVRSSNLLFMTRSSG
PTSVSADISGGSMQIAAAAQSAVSVAVNAEALIQEFDARAEQIRTARMKGYEGDACPECG
NFTLVRNGTCLKCDTCGGTTGCS