Protein Info for AMB_RS13620 in Magnetospirillum magneticum AMB-1

Annotation: acetyl-CoA carboxylase biotin carboxylase subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00514: acetyl-CoA carboxylase, biotin carboxylase subunit
  • amino acids 1 to 444 (444 residues), 732.9 bits, E=6.7e-225
• PF00289: Biotin_carb_N
  • amino acids 1 to 110 (110 residues), 159.1 bits, E=1.5e-50
• PF02786: CPSase_L_D2
  • amino acids 115 to 321 (207 residues), 274.5 bits, E=1.7e-85
• PF02222: ATP-grasp
  • amino acids 124 to 291 (168 residues), 36.7 bits, E=1e-12
• PF07478: Dala_Dala_lig_C
  • amino acids 125 to 290 (166 residues), 37 bits, E=8.1e-13
• PF02785: Biotin_carb_C
  • amino acids 335 to 440 (106 residues), 132 bits, E=2.8e-42

Best Hits

Swiss-Prot: 62% identical to ACCC_HAEIN: Biotin carboxylase (accC) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K01961, acetyl-CoA carboxylase, biotin carboxylase subunit [EC: 6.3.4.14 6.4.1.2] (inferred from 100% identity to mag:amb2708)

MetaCyc: 60% identical to biotin carboxylase (Escherichia coli K-12 substr. MG1655)
Biotin carboxylase. [EC: 6.3.4.14]

Predicted SEED Role

"Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14)" in subsystem Fatty Acid Biosynthesis FASII (EC 6.3.4.14)

MetaCyc Pathways

• biotin-carboxyl carrier protein assembly (4/4 steps found)
• superpathway of the 3-hydroxypropanoate cycle (13/18 steps found)
• 3-hydroxypropanoate cycle (9/13 steps found)
• glyoxylate assimilation (9/13 steps found)
• fatty acid biosynthesis initiation (type I) (1/3 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• jadomycin biosynthesis (3/9 steps found)
• superpathway of mycolate biosynthesis (21/239 steps found)

KEGG Metabolic Maps

• Fatty acid biosynthesis
• Propanoate metabolism
• Pyruvate metabolism
• Tetracycline biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 6.3.4.14, 6.4.1.2

Use Curated BLAST to search for 6.3.4.14 or 6.4.1.2

Sequence Analysis Tools

See Q2W3R3 at UniProt or InterPro

Protein Sequence (447 amino acids)

>AMB_RS13620 acetyl-CoA carboxylase biotin carboxylase subunit (Magnetospirillum magneticum AMB-1)
MFEKVLIANRGEIALRIHRACREMGIRTVAVHSTADNDAMHVRLADEAVCIGPPSARDSY
LNKAAILSAASITGADAIHPGYGFLSENADFAQMVEEHGFVFIGPTPDHIRMMGDKITAK
QAVKDAGIPVVPGSDGSVDTEETALEVAASIGYPVLIKATAGGGGKGMKVARNAEELVES
WKLARNEAKAAFGNADVYMEKYLGKPRHIEMQILADNYGAVVHLGERDCSLQRKHQKVLE
EAPSPALNADQRARIGKIACDAIAKLGYRNAGTIEFLYENGEFYFIEMNTRLQVEHPITE
AITGIDLVREQIRIAAGAPLGYTQADVRFAGHALECRVNAEDPVTFTPCPGRIEGYHAPG
GLGVRVDSGLYAGYRIPPHYDSMIAKLIVFGNTRNEALMRLKRALGEYVIEGVKTTLPLH
NRLVQDADFVNGDYDIHWLEKFVAQHS