Protein Info for AMB_RS13585 in Magnetospirillum magneticum AMB-1

Annotation: alpha/beta hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF12697: Abhydrolase_6
  • amino acids 5 to 223 (219 residues), 56.6 bits, E=1.2e-18
• PF00975: Thioesterase
  • amino acids 46 to 228 (183 residues), 33.8 bits, E=8.3e-12
• PF00561: Abhydrolase_1
  • amino acids 55 to 217 (163 residues), 46.9 bits, E=5.7e-16
• PF12146: Hydrolase_4
  • amino acids 56 to 211 (156 residues), 35.4 bits, E=1.5e-12

Best Hits

KEGG orthology group: None (inferred from 100% identity to mag:amb2701)

Predicted SEED Role

"Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24)" in subsystem Catechol branch of beta-ketoadipate pathway or Chloroaromatic degradation pathway or Protocatechuate branch of beta-ketoadipate pathway (EC 3.1.1.24)

MetaCyc Pathways

• protocatechuate degradation II (ortho-cleavage pathway) (2/4 steps found)
• catechol degradation to β-ketoadipate (1/4 steps found)
• catechol degradation III (ortho-cleavage pathway) (2/6 steps found)
• 4-methylcatechol degradation (ortho cleavage) (2/7 steps found)
• superpathway of salicylate degradation (2/7 steps found)
• aromatic compounds degradation via β-ketoadipate (3/9 steps found)
• toluene degradation III (aerobic) (via p-cresol) (4/11 steps found)
• mandelate degradation to acetyl-CoA (2/18 steps found)
• superpathway of aerobic toluene degradation (4/30 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (5/35 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.1.24

Sequence Analysis Tools

See Q2W3S0 at UniProt or InterPro

Protein Sequence (231 amino acids)

>AMB_RS13585 alpha/beta hydrolase (Magnetospirillum magneticum AMB-1)
MSRGLVLLPGLLNDHRLWLRQAAALGGEVEVLVADLTLDESLGAMAERVLGAAPERFALA
GLSMGGYVALEIMRRAPGRVERLALLDTTARPDLPEQTQRRRDAVELARSGGFDKIMPTM
LPLLLHPDHLADAAITALAKDMARAVGAQAFARQQAAIMARPDSRDSLAAIACPTLVLCG
AEDTLTPPDRHDEMAALIKGARRATIAHCGHLSPLEQPDAVSAELRAWLSV