Protein Info for AMB_RS13545 in Magnetospirillum magneticum AMB-1

Annotation: bifunctional diguanylate cyclase/phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 548 PF00990: GGDEF" amino acids 121 to 275 (155 residues), 141.6 bits, see alignment E=2.9e-45 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 122 to 277 (156 residues), 116 bits, see alignment E=1.5e-37 PF00563: EAL" amino acids 296 to 530 (235 residues), 245.1 bits, see alignment E=9.9e-77

Best Hits

KEGG orthology group: None (inferred from 100% identity to mag:amb2694)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W3S8 at UniProt or InterPro

Protein Sequence (548 amino acids)

>AMB_RS13545 bifunctional diguanylate cyclase/phosphodiesterase (Magnetospirillum magneticum AMB-1)
MNDIQALLNGLLDSINDAVIIADGDGNPLRSNPAFETLMGFTSKGGAEVSRLAAGMIYRA
INPGAGGRWRGLVTCPDGRERPFEVTAAAINPSTDSADRYLCVVRPAIAEEAGAAGGSFG
YDTLTGLPNRDLFGDRIGQAVLQANRTGASVALMTMGLDRFTLVNDALGHGAGDRLLMEV
ARRLKMCVRETDTAVRLDGDKFALVMAIADTDDSVIVAEKALNAVKEPFALDGQEVVVTF
SIGISVYPLDADSSAQLVKHAENALHYAKVSGRNQYQFFSKDMNRKAKSRLELEGRMRRA
LANQEFVVFYQPKVSADRDAIVGAEALIRWMDPERGMVPPGEFIPVAEESGQIEPIGTWV
LRQSCIQNRLWQEAGFNPIKVSVNVSARQFRSPSLLQTVTEVLDSTGLDPKWLELEITES
MLMNDVDTAVRKMKALRDLGIGLSIDDFGTGYSSLSYLGRFPITTLKIDRAFIADVDTNP
KTAEIARAIIGLSRGLNLEVVAEGAEIAEHIMFLRNNGCDTVQGFYYSRPVPADEFERMM
GQRVMAHA