Protein Info for AMB_RS13510 in Magnetospirillum magneticum AMB-1

Annotation: cytochrome C protein NapC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 213 transmembrane" amino acids 26 to 46 (21 residues), see Phobius details TIGR02161: periplasmic nitrate (or nitrite) reductase c-type cytochrome, NapC/NirT family" amino acids 12 to 195 (184 residues), 330 bits, see alignment E=2.1e-103 PF03264: Cytochrom_NNT" amino acids 22 to 193 (172 residues), 266.9 bits, see alignment E=1.5e-83

Best Hits

Swiss-Prot: 60% identical to NIRT_PSEST: Denitrification system component NirT (nirT) from Pseudomonas stutzeri

KEGG orthology group: K02569, cytochrome c-type protein NapC (inferred from 100% identity to mag:amb2686)

MetaCyc: 51% identical to NapC (Aliivibrio fischeri)
RXN-15816 [EC: 7.1.1.8]

Predicted SEED Role

"Cytochrome c-type protein NapC" in subsystem Nitrate and nitrite ammonification or trimethylamine N-oxide (TMAO) reductase

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.1.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W3T5 at UniProt or InterPro

Protein Sequence (213 amino acids)

>AMB_RS13510 cytochrome C protein NapC (Magnetospirillum magneticum AMB-1)
MSTENPAKQGVLSGWWATLRRPSSRWSLGLLLVVGFVGGVMFWGGFNTVLELTNTEAFCL
SCHEMEQNVYREYRGTIHDSNRSGVRATCPDCHVPRPWIYKIQRKIQASNEVWHKILGSI
DTREKFEAKRIELAKHEWARMKSTDSRECRNCHNFGAFDLTRQQARARNRHSEAEQQGKT
CIDCHKGIAHQLPAGAFRAERELNESVGGAVKP