Protein Info for AMB_RS13440 in Magnetospirillum magneticum AMB-1

Annotation: formate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 961 PF04879: Molybdop_Fe4S4" amino acids 70 to 121 (52 residues), 37.5 bits, see alignment 2.8e-13 PF00384: Molybdopterin" amino acids 125 to 566 (442 residues), 168.6 bits, see alignment E=3.1e-53 PF01568: Molydop_binding" amino acids 826 to 952 (127 residues), 71 bits, see alignment E=1.3e-23

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 74% identity to azl:AZL_007310)

Predicted SEED Role

"Formate dehydrogenase-O, major subunit (EC 1.2.1.2)" in subsystem Formate hydrogenase (EC 1.2.1.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W3V0 at UniProt or InterPro

Protein Sequence (961 amino acids)

>AMB_RS13440 formate dehydrogenase (Magnetospirillum magneticum AMB-1)
MLIKKRDGEARKSALSENLASVSGGSMDRRAFLRRSGLTAGGAALLGGIGAGTVRKADAV
PVAPDAKAEVKRAICTHCSVGCTVNAEVSNGVWIGQEPAFDSPLNLGSHCAKGAAVREHA
HGDRRLKYPMKLEGGKYKRISWEQAITEVGDRLLKIRETAGPDSVYWLGSAKFSNEQAYL
MRKFAALWGTNNVDHQARICHSTTVAGVAQTWGYGAMTNSYNDIHNSKAMFFIGSNAAEA
HPVAMLHILKAKEQNKAPLIVCDPRFTRTAAHADEYVRMRPGTDVPLIWGIMWHIFENGW
EDKEFIRKRVWGMDQVKAEVAKWTPEETERVTGVPGAQLKRVAQILATHKPGTVVWCMGG
TQHTVGNANVRAYCNLQLALGNVGVAGGGTNIFRGHDNVQGATDMGLDVTTLPAYYGLAA
PAWKHWARVWGLDYDYIKGRFKTPELMEAPGIPVSRWYDGVNEAKENIAQPEPIKAMVYW
GHAPNSQTRQPELKKAMEKLDTLVIIDPYPTVSAVLHDRTDGVYLLPAATQFEVDGSRTA
SNRSIQWSEQVIKPMFECKADQEIMYLFAKKFGYAAELCKNIKVEGDKPVAEDILREINR
GAWTIAYTGQSPERLKAHMANQQTFDKTTLRAVGGPCDGDYYGLPWPCWGTAEMKHPGTP
NLYDPSKPVKDGGLNFRALFPEYKGESLLATDSWPEGSELPAGHPGFTYGMLKKLGWDVD
LTPEEKATIEKIGGEKADDVAWTIDLSGGIQRVAVKHGAAPFGNGKARAVVWNFPDPVPL
HREPLYTPRRDLVEKYPTHADKKMMRLPFLYKSIQDKDVSKEFPIILTSGRLVEYEGGGD
ETRSNPWLAELQPEMFVEVNPFDANNAGIRAGDYVWVHGPEGGKVKVKARITERVGRGVA
FMPFHFGGWFQGKDLRSKYPEGASPIVLGESTNTAQTYGYDSVTGMQETKVTLCRIEKFQ
A