Protein Info for AMB_RS13390 in Magnetospirillum magneticum AMB-1

Annotation: fructose 1,6-bisphosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 338 PF00316: FBPase" amino acids 6 to 194 (189 residues), 216 bits, see alignment E=3.6e-68 PF18913: FBPase_C" amino acids 198 to 331 (134 residues), 162.1 bits, see alignment E=5.8e-52

Best Hits

Swiss-Prot: 100% identical to F16PA_MAGSA: Fructose-1,6-bisphosphatase class 1 (fbp) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K03841, fructose-1,6-bisphosphatase I [EC: 3.1.3.11] (inferred from 100% identity to mag:amb2661)

MetaCyc: 43% identical to fructose-1,6-bisphosphatase 2 (Homo sapiens)
Fructose-bisphosphatase. [EC: 3.1.3.11]

Predicted SEED Role

"Fructose-1,6-bisphosphatase, type I (EC 3.1.3.11)" in subsystem Calvin-Benson cycle or Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes (EC 3.1.3.11)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.11

Use Curated BLAST to search for 3.1.3.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W3W0 at UniProt or InterPro

Protein Sequence (338 amino acids)

>AMB_RS13390 fructose 1,6-bisphosphatase (Magnetospirillum magneticum AMB-1)
MPYKRITLTHFLLQEQRRLGGSGSFTALMTDIIFACKMISHEVNRGALAGNLGVAGSENV
QGEEQKKLDVLANDIFLHMNALGGSYAGMASEELEDVHAVHGAADGKYLLLFDPLDGSSN
IDVNISVGSIFSILKLPEGANAGSKDAFLQPGVKQVAAGYALYGSSTMLVLTTGNGVNGF
TLDNNVGMFLLTHPNMTIPADTKEFAINASRERFWEPPVKRYIDECRQGKEGPRGKDFNM
RWVASMVAEVHRILCRGGVFLYPADTENMKKGGKLRLMYEANPMAFIVEQAGGAATTGRG
RMMEVPPTGLHQRVPVILGSKEEVERIGAYHAEYDAKK