Protein Info for AMB_RS13335 in Magnetospirillum magneticum AMB-1

Annotation: glycine--tRNA ligase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 688 TIGR00211: glycine--tRNA ligase, beta subunit" amino acids 3 to 686 (684 residues), 610 bits, see alignment E=4.2e-187 PF02092: tRNA_synt_2f" amino acids 4 to 543 (540 residues), 658.1 bits, see alignment E=1.3e-201 PF05746: DALR_1" amino acids 574 to 674 (101 residues), 33.9 bits, see alignment E=2.9e-12

Best Hits

Swiss-Prot: 100% identical to SYGB_MAGSA: Glycine--tRNA ligase beta subunit (glyS) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K01879, glycyl-tRNA synthetase beta chain [EC: 6.1.1.14] (inferred from 100% identity to mag:amb2650)

Predicted SEED Role

"Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W3X1 at UniProt or InterPro

Protein Sequence (688 amino acids)

>AMB_RS13335 glycine--tRNA ligase subunit beta (Magnetospirillum magneticum AMB-1)
MAEFLLEIFSEEIPARMQARAADDLRGMVSDGLAKNGITFDAARSYVTPRRLVLVIEGLP
LAGPDVSEEKRGPRVSSPAQAMEGFLKSTGMTVEQLEQRDTGKGVFYFAVINRKGRPTAE
VLREVTEGAMATFPWPKSQRWGANSIRWVRPIQSILALFDGSIVPVAFGPATAGDMTAGH
RFLAPEPFRVKDFADYLSKLRHAKVMLDPVERRHAILAGAEGLAAAEGLQLKADDGLLAE
VTGLVEWPVPLVGSIDDKFMDVPAEVLITSMRAHQKYFSLLKADGSLAPRFIVISNMETD
DGGKAVVAGNQRVLRARLSDAKFFWDTDRKQRLEARLPKLAERTFYASLGTVADKVDRIS
ALAGNIAGMIGADRAVAERAARLAKADLSTELVGEFPELQGLMGRYYALNDGETPEVADA
IAAHYSPQGPGDSCPTAPVSVAVALADKIDSLVGFFAINEKPTGSKDPFALRRAALGIIR
LVLENGLRLPLSQVIFLASGHYKANLVNSPGEVANDLLNFFADRLAVALKEKGVRHDLIT
AIFSLGGEDDLVRLLKRVEALGAFLDSDDGANLLIAYRRAANIVRIEEKKDGTIFSGYAD
IQLLRQDEEKALEHALSDVGQAVSNALVTEDFAAAMAALARLRRPVDAFFDKVTVNADEA
PLRVNRLKLLAMIGSAMGRLADFSKVEG