Protein Info for AMB_RS13195 in Magnetospirillum magneticum AMB-1

Annotation: putrescine ABC transporter permease PotI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 268 transmembrane" amino acids 7 to 31 (25 residues), see Phobius details amino acids 67 to 88 (22 residues), see Phobius details amino acids 98 to 123 (26 residues), see Phobius details amino acids 136 to 157 (22 residues), see Phobius details amino acids 178 to 202 (25 residues), see Phobius details amino acids 210 to 226 (17 residues), see Phobius details amino acids 238 to 261 (24 residues), see Phobius details PF00528: BPD_transp_1" amino acids 76 to 255 (180 residues), 46.4 bits, see alignment E=1.9e-16

Best Hits

Swiss-Prot: 61% identical to POTI_ECOL6: Putrescine transport system permease protein PotI (potI) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K11074, putrescine transport system permease protein (inferred from 100% identity to mag:amb2622)

MetaCyc: 61% identical to putrescine ABC transporter membrane subunit PotI (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Putrescine transport system permease protein PotI (TC 3.A.1.11.2)" in subsystem Polyamine Metabolism (TC 3.A.1.11.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W3Z9 at UniProt or InterPro

Protein Sequence (268 amino acids)

>AMB_RS13195 putrescine ABC transporter permease PotI (Magnetospirillum magneticum AMB-1)
MKNRGFALYTALALVYAFLYLPIALLVIYSFNASKLVTVWGGFSTKWYGELLHDAQVLDA
AWLSLKIAFLNACLATALGTMAAVVLVRFRRFSGRALFTGMVSAPLVMPEIITGLALLLL
FVGMENLLGWPDGRSMTTIVIAHVTFSLSFVTVVVRSRLAQMDLSLEEAAMDLGARPLTV
FFSITLPIIAPALAAGWLLAFTLSVDDVVISAFVSGPGATPLPIVIFSKVRLGVSPEINA
LATIVVALVGVAIAVAGRVMMTREKQGG