Protein Info for AMB_RS13010 in Magnetospirillum magneticum AMB-1

Annotation: DNA topoisomerase IV subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 744 TIGR01062: DNA topoisomerase IV, A subunit" amino acids 13 to 744 (732 residues), 848.9 bits, see alignment E=1.5e-259 PF00521: DNA_topoisoIV" amino acids 34 to 471 (438 residues), 503.6 bits, see alignment E=4.9e-155 PF03989: DNA_gyraseA_C" amino acids 609 to 645 (37 residues), 9.7 bits, see alignment 6.4e-05 amino acids 658 to 692 (35 residues), 11.7 bits, see alignment (E = 1.4e-05)

Best Hits

Swiss-Prot: 60% identical to PARC_RHIME: DNA topoisomerase 4 subunit A (parC) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K02621, topoisomerase IV subunit A [EC: 5.99.1.-] (inferred from 100% identity to mag:amb2585)

Predicted SEED Role

"Topoisomerase IV subunit A (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W436 at UniProt or InterPro

Protein Sequence (744 amino acids)

>AMB_RS13010 DNA topoisomerase IV subunit A (Magnetospirillum magneticum AMB-1)
MTKPAVTGDIRDTPLAEALGERYLAYAMSTIVSRSLPDVRDGLKPVHRRLLFAMRQLKLD
PSGGFKKCARVVGDVIGKYHPHGDVAVYDTLVRLAQDFAVRYPLVEGQGNFGNIDGDNAA
AMRYTEAKLTEIAAALMEGLDEDSVDFRPTYDGTEEEPVVMPSAFPNLLANGSAGIAVGM
ATSIPPHNIGEICDALDHLIAKPDCDVDELIAHMPGPDFPTGGVLVETQAAITEAYRTGK
GAFRVRAKWAVEKLSHGLYQIVITEIPYQVQKAKLIEKIAELLSEKKLPLLADIRDESAE
DIRLVLEPRNRTVEAELVMEQLFRQTDLESRFSLNMNVLAPDAHGHLTPGVMNLKEVLRA
FLDHRMVVLERRARFRLEKIAKRLEVLEGFLKVYADLDKVIRIIRFADEPKAELIKTFKL
SEVQAEAILNMRLRSLNKLQELEIKTEHAELSAEKADLEALLADEPRRWAAIAAQNDETR
KAFGGATALGKRRTELGDAPSAVVVPIEAYVEREAITMVLSEKGWIRALKGHSDAPEALK
FKEGDQLGHLLKAWTTDKILVFATDGRFYTIGGDKLPSGRGHGEPLRLMIDLAGDVDIAA
VMVHKPGRKLLVATNTDRGFVVEENEVLAQTRAGKMVLNCADGEKAKFCLPFEGDAVAVI
GENRKLLVFMAEEIPVLTRGRGVILQKYRDGGMADIKVFTLAQGLTWNLGDRVRTESDLT
PWLGKRASVGRLPPTGFPRNNRFS