Protein Info for AMB_RS12730 in Magnetospirillum magneticum AMB-1

Annotation: arginine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 576 transmembrane" amino acids 550 to 572 (23 residues), see Phobius details PF03485: Arg_tRNA_synt_N" amino acids 7 to 88 (82 residues), 72.7 bits, see alignment E=9.3e-24 TIGR00456: arginine--tRNA ligase" amino acids 10 to 575 (566 residues), 362.7 bits, see alignment E=1.5e-112 PF00750: tRNA-synt_1d" amino acids 98 to 421 (324 residues), 63.3 bits, see alignment E=6.2e-21 PF05746: DALR_1" amino acids 446 to 575 (130 residues), 87.3 bits, see alignment E=2.6e-28

Best Hits

Swiss-Prot: 100% identical to SYR_MAGSA: Arginine--tRNA ligase (argS) from Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)

KEGG orthology group: K01887, arginyl-tRNA synthetase [EC: 6.1.1.19] (inferred from 100% identity to mag:amb2529)

Predicted SEED Role

"Arginyl-tRNA synthetase (EC 6.1.1.19)" (EC 6.1.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W492 at UniProt or InterPro

Protein Sequence (576 amino acids)

>AMB_RS12730 arginine--tRNA ligase (Magnetospirillum magneticum AMB-1)
MNLFKYFREEIIKSVEAIVPGLDTSKVTAEPPREVSHGDVATNAAMVLTKAAGMKPRDLA
EKIAEVLRAHPAVTGVEVAGPGFINLRLADSFWFERLAEVLAAGTAYGQSDLGRGHKVNV
EYVSANPTGPMHIGHARGAVFGDALASLLVKAGYDVTREYYVNDAGSQVDVLARSAFLRY
REALGEEIGEIPEGLYPGEYLKPAGEALAKKHGPALKDKPEEEWLPELRGFAVDAMLDMI
KGDLAGLGVRHDVFVSERGLVEAGKVQDALDYLTAKGLIYEGVLEPPKGKLPDDWEARPQ
TLFKATEFGDDVDRPLKKSDGSWTYFASDIAYHLDKYRRGFGAMIDVWGADHGGYVKRMQ
AAVKAVSEGGGDLDVKLCQMVNLLKGGQPYKMSKRAGTFVTLRDLVEAVGKDVVRFIMLT
RKNDAHLDFDLDKVLEQSRDNPVFYVQYAHARCHSVMRHAAGMWPDAVGPVPGVEVLAHL
TDPAELGLIRLLAGWPRLVESAAEAHEPHRVAFYLYDLAAAFHGLWNKGKDDTSLRFLIE
DDRELSLARLGLLRAVVGVIASGLGIFGVAPVEEMR