Protein Info for AMB_RS12640 in Magnetospirillum magneticum AMB-1

Annotation: protein translocase subunit SecF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 319 transmembrane" amino acids 21 to 40 (20 residues), see Phobius details amino acids 142 to 159 (18 residues), see Phobius details amino acids 166 to 207 (42 residues), see Phobius details amino acids 240 to 262 (23 residues), see Phobius details amino acids 271 to 295 (25 residues), see Phobius details PF07549: Sec_GG" amino acids 37 to 59 (23 residues), 30.2 bits, see alignment (E = 2.4e-11) TIGR00966: protein-export membrane protein SecF" amino acids 42 to 288 (247 residues), 259.4 bits, see alignment E=3.8e-81 TIGR00916: protein-export membrane protein, SecD/SecF family" amino acids 89 to 285 (197 residues), 183 bits, see alignment E=7e-58 PF02355: SecD_SecF" amino acids 116 to 296 (181 residues), 219.3 bits, see alignment E=3.1e-69

Best Hits

Swiss-Prot: 47% identical to SECF_RICFE: Protein translocase subunit SecF (secF) from Rickettsia felis (strain ATCC VR-1525 / URRWXCal2)

KEGG orthology group: K03074, preprotein translocase subunit SecF (inferred from 100% identity to mag:amb2513)

MetaCyc: 39% identical to Sec translocon accessory complex subunit SecF (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Protein-export membrane protein SecF (TC 3.A.5.1.1)" (TC 3.A.5.1.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W4A8 at UniProt or InterPro

Protein Sequence (319 amino acids)

>AMB_RS12640 protein translocase subunit SecF (Magnetospirillum magneticum AMB-1)
MQLIDRFLPHGTKYDFIRYRWFAFCITAVLIIGSFASIAIKGFNFGIDFAGGILLEAQST
TGPADLHGMRTTLGHLGLGEVSLQEFGTTGRDVMIRVQRQEGDEKTQMAALSKVKASLGE
GFTYRRVEIVGPKVGGELVRDGVLAVVLALGAIALYVWFRFEWQFGVGALVSTFHDVITT
FGLFSITGLEFNLTTVAAILTIAGYSVNDTVVEYDRVRENLRKFKTMSLYDLLNLAVNET
LARTILTVSTVFVTVLALLFVGGEVLRGFSIAMLWGLIIGTFSSIYVAMPMLIYFNLRTG
KAAEEAEAEEAPTGGPAAP