Protein Info for AMB_RS11400 in Magnetospirillum magneticum AMB-1

Annotation: bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 721 TIGR00691: RelA/SpoT family protein" amino acids 26 to 715 (690 residues), 721 bits, see alignment E=7.5e-221 PF13328: HD_4" amino acids 26 to 176 (151 residues), 157.8 bits, see alignment E=5.8e-50 PF01966: HD" amino acids 45 to 144 (100 residues), 37.2 bits, see alignment E=9.9e-13 PF04607: RelA_SpoT" amino acids 236 to 345 (110 residues), 146.8 bits, see alignment E=8.8e-47 PF02824: TGS" amino acids 391 to 449 (59 residues), 74.1 bits, see alignment 2.2e-24 PF19296: RelA_AH_RIS" amino acids 460 to 631 (172 residues), 141.9 bits, see alignment E=8e-45 PF13291: ACT_4" amino acids 646 to 718 (73 residues), 63.4 bits, see alignment E=7.5e-21

Best Hits

KEGG orthology group: None (inferred from 100% identity to mag:amb2253)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W518 at UniProt or InterPro

Protein Sequence (721 amino acids)

>AMB_RS11400 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (Magnetospirillum magneticum AMB-1)
MMRQFELVERVKSYDPNADEDAINRAYVFAMKMHGSQLRASGDPYFSHPIEVAGILTKYR
LDSASIITALLHDTIEDTPATLDDIEKLFGREIGRLVDGVTKLNRIELQSDHAKQAENLR
KLVLAMSEDIRVLLVKLADRMHNMRTLHYIKNPDKRRRIAMETMEIYAPLAERIGMQGIK
MELEDLAFAELHPDARGSIVARLSFLREQGGDLIGRILDELRSILAEPGIKAVVSGREKT
PYSIWQKMQRKNVGFEQLSDIMAFRVAVDNVEDCYRALGVIHSKYPMVPNRFKDYISTPK
PNGYRSLHTGVFGPERHRIEVQIRTSEMHEVAELGVAAHWKYKGGGGEGGNMTDGRQYRW
LRELLDILEHASNPEEFLEHTKLEMFSDQVFCFTPKGDLISLPRGACPVDFAYAVHSQVG
DTCVGAKVNGRIMPLRTQLQNGDQVDIITSKAQTPNPTWERFVVTGKARARIRRFIRTQQ
RAQYTELGRAILVRSFRQEGYEFTEKALEGVLRIFKAPSAEDLLALVGEGTLTAREVVST
VFPELKAQANRNDNVVALKVRGDKPSTNKKDKAGAVPIKGLIPGMAMHFAGCCHPLPGDR
IVGIVSTGKGVTIHTIDCENLEQFAEAPERWLDLAWDEADSAAHVGRIDLVVTNEPGAFG
AISTVIAKNMGNITNLKITNRTTDFFEMLIDIEVRDVKHLTNVIAALRATPAINSVDRAR
N