Protein Info for AMB_RS11370 in Magnetospirillum magneticum AMB-1

Annotation: valine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 889 transmembrane" amino acids 481 to 499 (19 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 5 to 867 (863 residues), 1113.6 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 17 to 569 (553 residues), 497.6 bits, see alignment E=6.8e-153 PF09334: tRNA-synt_1g" amino acids 42 to 95 (54 residues), 28.5 bits, see alignment 1.7e-10 amino acids 526 to 574 (49 residues), 27.7 bits, see alignment 3e-10 PF08264: Anticodon_1" amino acids 612 to 757 (146 residues), 130.4 bits, see alignment E=1.3e-41 PF10458: Val_tRNA-synt_C" amino acids 822 to 886 (65 residues), 63.7 bits, see alignment 4.1e-21

Best Hits

Swiss-Prot: 59% identical to SYV_ZYMMO: Valine--tRNA ligase (valS) from Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)

KEGG orthology group: K01873, valyl-tRNA synthetase [EC: 6.1.1.9] (inferred from 78% identity to azl:AZL_013080)

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2W524 at UniProt or InterPro

Protein Sequence (889 amino acids)

>AMB_RS11370 valine--tRNA ligase (Magnetospirillum magneticum AMB-1)
MAMLDKTYRPAEVEPKHYERWEAQGAFAAHTDSNAAPYTIMMPPPNVTGSLHMGHALTFT
LQDVLIRYRRMTGKDALWQPGTDHAGIATQMVVERQLEAQKVTRHDLGRDNFIKRVWEWK
AESGGTITRQLRRLGASPDWAKERFTMDEGLSAAVRKVFVTLHRQGLIYRAKRLVNWDPK
LHTAISDLEVEQREVKGHMWHFRYPVEGMADTFITVATTRPETMLGDAAVAVHPEDERFA
ALVGKMVRLPIVNRLIPIVADEYSDPTKGTGAVKITPAHDFNDFQVGVRHDLPQINIFDR
DARTIDEVPEGYRGLDRYDARKKVVEEFEALGLLDKIEPHTHMVPYGDRSGVVIEPWLTD
QWFVDAATLAKPAIEAVETGKTRFVPKHWENTYFEWMRNIQPWCISRQIWWGHQVPAWYG
PDGAFFVEETEDEARAAAAAHYGKDVELTRDSDVLDTWFSSALWPFSTLGWPEKTPELDR
YYPGDVLVTGFDIIFFWVARMMMMGIHFMGDVPFRDIYIHALVRDEKGQKMSKSKGNIID
PLDLIEKYGCDALRFTLSALAAQGRDVKLAESRVEGYRNFATKLWNAARFCQMNECRPVA
GFDPKAVKETVNRWIVAKTAELAAKVGTAIEGYRYDAAAGGAYQFVWGTFCDWYLEFAKP
IFNGGDEAAKAETRATAAWVLDQILHVLHPLMPFITEELWGQIAERDGDLMLRSWPALDG
LAAPEAEEEMDWVVRVISTVRGVRAEMNVPPSAQVDLLVSGLAETKRGWAKTHADLITRL
VRLTAFDAQATPERVAQASSHGAAQMVVDEATLVMPLGGVIDVDKERSRLDKEIARLEGE
IAKVDKKLGNADFVAKARPEVVEEQHERRADWAGAVDKLREALERLSGA