Protein Info for AMB_RS11200 in Magnetospirillum magneticum AMB-1

Annotation: adenylyl-sulfate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00231: small GTP-binding protein domain
  • amino acids 22 to 185 (164 residues), 43.8 bits, E=3.5e-15
• PF00009: GTP_EFTU
  • amino acids 23 to 205 (183 residues), 154.5 bits, E=5.1e-49
• TIGR02034: sulfate adenylyltransferase, large subunit
  • amino acids 24 to 422 (399 residues), 338.3 bits, E=1e-104
• TIGR00455: adenylyl-sulfate kinase
  • amino acids 446 to 623 (178 residues), 234.3 bits, E=1.2e-73
• PF01583: APS_kinase
  • amino acids 459 to 611 (153 residues), 222.3 bits, E=6.1e-70
• PF13671: AAA_33
  • amino acids 461 to 554 (94 residues), 27.5 bits, E=7e-10

Best Hits

KEGG orthology group: K00955, bifunctional enzyme CysN/CysC [EC: 2.7.1.25 2.7.7.4] (inferred from 100% identity to mag:amb2212)

Predicted SEED Role

"Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25)" in subsystem Cysteine Biosynthesis or O-Methyl Phosphoramidate Capsule Modification in Campylobacter (EC 2.7.1.25, EC 2.7.7.4)

MetaCyc Pathways

• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• sulfate activation for sulfonation (2/2 steps found)
• assimilatory sulfate reduction IV (3/4 steps found)
• sulfite oxidation III (2/3 steps found)
• dissimilatory sulfate reduction I (to hydrogen sufide)) (3/5 steps found)
• superpathway of sulfide oxidation (phototrophic sulfur bacteria) (8/12 steps found)
• assimilatory sulfate reduction II (1/3 steps found)
• superpathway of thiosulfate metabolism (Desulfovibrio sulfodismutans) (3/6 steps found)
• selenate reduction (2/5 steps found)
• superpathway of sulfur metabolism (Desulfocapsa sulfoexigens) (2/6 steps found)
• superpathway of seleno-compound metabolism (5/19 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.4

Use Curated BLAST to search for 2.7.1.25 or 2.7.7.4

Sequence Analysis Tools

See Q2W559 at UniProt or InterPro

Protein Sequence (641 amino acids)

>AMB_RS11200 adenylyl-sulfate kinase (Magnetospirillum magneticum AMB-1)
MSNTATALKAIPEVSVSHTASPMKIVVVGHVDHGKSTLVGRILNETGALPEGKVEYLREV
CDKRGMPFEWAFVMDALQAERDQGITIDTAQIRFKTEARPYVIIDAPGHKEFLKNMVSGA
ASAEAAVLVIDAHEGVQEQSRRHGYLLHLLGLRQIAVAVNKMDLVNFSESRFEEVKAEIV
AYLNSIGVEPTYVVPVAARSGANIATKSVETPWYQGPSVLGALDMFEPAKPATDLALRFP
VQDVYKFDERRIIAGRIESGRLKVGDTLVFAPSGKMAKVATIETWGNVAAAVSAGAGQSV
GITLDEQIFVERGNVASLEHHVPHQSHDLKARVFWLGRGPLKVGSRYKLKLATAEHVVDV
VAIERVIDVEDLANHQGTEVARNAVAEVVFRGKSPIAHDSFIDNPRLGRFVLVEGYDIVG
GGVIAEASSEDARNRSTHVTEVPHKVDAETRSIANGHRGGVVWLTGLSGAGKSTIAMEVE
RQLFLKGWQVTVLDGDNVRTGLCGDLGFSDADRAENIRRVGEVAHLFADAGMVVVTSFIS
PFRADREKVRAINPDAFHEVHVATGLNECERRDPKGLYKKARSGEISYFTGVSSAYEAPA
LPELTLATEGKSVDESVADLLRYIDKAFSLSAREQANGWGL