Protein Info for AMB_RS10805 in Magnetospirillum magneticum AMB-1

Annotation: benzoyl-CoA reductase subunit D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02261: benzoyl-CoA reductase, subunit D
  • amino acids 5 to 265 (261 residues), 465.7 bits, E=3.6e-144
• TIGR00241: putative CoA-substrate-specific enzyme activase
  • amino acids 5 to 263 (259 residues), 152.7 bits, E=1.3e-48
• PF01869: BcrAD_BadFG
  • amino acids 8 to 265 (258 residues), 232.8 bits, E=2.8e-73

Best Hits

Swiss-Prot: 74% identical to BCRD_THAAR: Benzoyl-CoA reductase subunit D (bcrD) from Thauera aromatica

KEGG orthology group: K04115, benzoyl-CoA reductase subunit [EC: 1.3.7.8] (inferred from 100% identity to mag:amb2138)

MetaCyc: 74% identical to benzoyl-CoA reductase delta subunit (Thauera aromatica)
1.3.99.15-RXN [EC: 1.3.7.8]

Predicted SEED Role

"Benzoyl-CoA reductase subunit BadG (EC 1.3.99.15)" (EC 1.3.99.15)

MetaCyc Pathways

• benzoyl-CoA degradation II (anaerobic) (3/7 steps found)
• benzoyl-CoA degradation III (anaerobic) (2/9 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (7/17 steps found)
• toluene degradation VI (anaerobic) (6/18 steps found)
• anaerobic aromatic compound degradation (Thauera aromatica) (11/27 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation

Isozymes

Compare fitness of predicted isozymes for: 1.3.7.8, 1.3.99.15

Use Curated BLAST to search for 1.3.7.8 or 1.3.99.15

Sequence Analysis Tools

See Q2W5D3 at UniProt or InterPro

Protein Sequence (279 amino acids)

>AMB_RS10805 benzoyl-CoA reductase subunit D (Magnetospirillum magneticum AMB-1)
MSELITTGVDIGSGCIKTVVFKVNGDKVEWLGKETARIRNRDPFQLTNEAYDHMLKTAGV
DRKDVAYVASTGDAENLSFATGHFYSMTTHGRGGLYLNPEARAVLDIGALNGRAIRMDGV
GKVLSYKMTSQCASGSGQFLENIARYLGIAQDEIGSLSMKADDPEKVSSICAVLAETDVI
NMVSRGISASNILKGIHVSMAVRLAKLLKSIGAVDGIVQVTGGLALDTGLVAALNEAAEQ
EKVNLKAVSHPDSIYAGAIGAALWGAFRHEKLARLGQAA